Exocytic Event Classification
Exocytic Event Classification - Related Videos
03:54
Total Internal Reflection Fluorescence Microscopy for Visualization of Exocytic Events
0 Views •
2025
Source: Winkle, C. C., et al. Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis. J. Vis. Exp. (2016)This video demonstrates total internal reflection fluorescence (TIRF) microscopy for visualizing exocytic events in cortical neurons. A pH-sensitive green fluorescent protein (GFP) marker fluoresces upon vesicle fusion, with selective TIRF excitation capturing membrane-proximal exocytosis in time-lapse imaging.
14:33
Event-related Potentials and the Oddball Task
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2023
Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California Given the overwhelming amount of information captured by the sensory organs, it is crucial that the brain is able to prioritize the processing of certain stimuli, to spend less effort on what might not be currently important and to attend to what is. One heuristic the brain uses is to ignore stimuli that are frequent or constant in favor of stimuli that are unexpected or unique. Therefore, rare events...
09:00
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
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Cited by 13 •
2013
Time-lapse microscopy of fluorescently labeled autophagy markers allows monitoring of the dynamic autophagy response with high temporal resolution. Using specific autophagy and organelle markers in a combination of 3 different colors, we can follow the contribution of a protein to autophagosome formation in a robust spatial and temporal context.
01:28
Integration of Synaptic Events
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2025
Synaptic integration mainly includes the summation of graded potentials. Graded potentials, regardless of their type, cause subtle alterations in membrane voltage, resulting in either depolarization or hyperpolarization. These incremental changes, when combined or summed, can propel the neuron toward its threshold. Consider, for example, a membrane experiencing a +15 mV shift, causing it to depolarize from -70 mV to -55 mV. In this scenario, graded potentials govern the membrane's ability to...
01:30
Classification of Leukocytes
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2025
Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm. Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
FAQs
Imaging signals and membrane changes help distinguish two major outcomes. When the fusion pore expands, the vesicle proceeds toward full collapse, whereas pore closure indicates a transient kiss-and-run event. This classification links visible cellular changes to the route of vesicle release, rather than treating all exocytic events as equivalent.
The fusion pore marks a critical transition in membrane fusion. Its behavior determines whether release proceeds through pore expansion or a temporary opening followed by closure. In classification, this distinction connects the physical membrane pathway with cargo discharge and indicates whether the vesicle follows a recycling behavior associated with transient release.
By grouping events according to their release behavior, researchers can compare how often vesicles release, when release occurs, how cargo is discharged, and whether vesicles show different recycling behavior. These measurements help identify distinct secretory patterns within a cell or across biological systems, without assuming that every fusion event has the same outcome.
A basic analysis starts by identifying a vesicle that docks and fuses with the plasma membrane. Researchers then examine imaging signals and membrane changes to determine whether the fusion pore expands or closes again. Grouping events by these observed outcomes allows comparison of release timing, cargo discharge, and recycling behavior.
The approach supports studies of neurotransmission, hormone secretion, and immune-cell signaling, where vesicle release must be compared across events. It also helps investigate vesicle trafficking by linking membrane-fusion outcomes with recycling behavior. These applications connect event-level observations with regulated secretion and cellular communication.
Distinguishing full collapse from kiss-and-run allows researchers to interpret secretion as a set of mechanistically different events rather than one uniform process. The resulting patterns can be related to release probability, timing, cargo discharge, and recycling behavior, providing a framework for studying how membrane fusion regulates signaling and vesicle trafficking.