Gastrointestinal Biopsy
Gastrointestinal Biopsy - Related Videos
06:02
Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens
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Cited by 17 •
2016
Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.
08:15
Gastrointestinal Motility Monitor (GIMM)
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Cited by 36 •
2010
Evaluation of colonic motility in the guinea pig distal colon with the Gastrointestinal Motility Monitor (GIMM) is a straightforward and simple to learn approach to quantitatively evaluate propulsive motility in the gastrointestinal tract.
08:08
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
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Cited by 4 •
2014
This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.
01:26
Anatomy of the Gastrointestinal System
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2025
The human digestive system is an intricate and essential network for nutrient absorption and waste elimination. It encompasses the gastrointestinal (GI) tract and several accessory organs. Here's a detailed walkthrough of this complex system: Oral Cavity: The starting point of the digestive process. It comprises lips, cheeks, teeth, and salivary glands within mucous membranes. It extends to the soft palate, the floor of the mouth, and the tongue. Pharynx: It is a tubular...
06:04
Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
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Cited by 41 •
2016
This protocol describes the use of excised intestinal tissue preparations or "intestinal sacs" as an ex vivo model of intestinal barrier function. This model may be used to assess integrity of both the epithelial barrier and the mucous gel layer at specific intestinal sites in animal models of digestive disease.
FAQs
Microscopic and molecular examination provide complementary views of the same specimen. Microscopy reveals changes in cell structure, inflammation, infection, or malignancy, whereas molecular examination extends analysis beyond visible tissue features. Using both approaches can connect observable structural abnormalities with molecular findings, making biopsy useful for diagnostic evaluation and investigating how gastrointestinal disease develops.
Routine imaging and endoscopy primarily provide views of gastrointestinal structures, while biopsy analysis examines the tissue itself. This difference matters when abnormal cell structure, inflammation, infection, or malignancy is not visibly apparent. Tissue-level findings can therefore add diagnostic information that visual examination alone may not provide.
Preservation keeps collected tissue suitable for later analysis, sectioning creates thin portions that can be examined, and staining makes structural features easier to evaluate under microscopy. Together, these preparation steps convert a specimen into an interpretable laboratory record, supporting assessment of cell structure and changes associated with inflammation, infection, or malignancy.
During the collection workflow, an endoscope guides forceps to a selected digestive-tract location, such as the esophagus, stomach, small intestine, or colon. The retrieved tissue is then preserved, sectioned, stained, and examined. This sequence links accurate sampling with standardized laboratory preparation, allowing the final specimen to support microscopic and molecular analysis.
In clinical work, findings from these specimens can help evaluate celiac disease, inflammatory bowel disease, and gastrointestinal cancer. In biology and clinical research, the same material supports investigation of tissue function, disease mechanisms, and treatment response. Its value therefore extends from identifying pathology to examining how digestive-tract tissues behave during disease or therapy.
Because the specimen permits direct examination of tissue, investigators can relate cell-structure changes to broader biological questions about inflammation, infection, malignancy, and tissue function. Molecular examination adds another layer to this analysis. This makes biopsy material useful not only for identifying abnormality, but also for investigating disease processes and evaluating treatment response.