Newcastle Disease Virus
Newcastle Disease Virus - Related Videos
10:55
Rescue of Recombinant Newcastle Disease Virus from cDNA
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Cited by 25 •
2013
Newcastle disease virus (NDV) has been extensively studied in the last few years in order to develop new vectors for vaccination and therapy, among others. These studies have been possible due to techniques to rescue recombinant virus from cDNA, such as those we describe here.
07:24
Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
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Cited by 3 •
2022
Here we provide a detailed procedure for production, purification, and quantification of high-titer recombinant Newcastle disease virus. This protocol consistently yields > 6 × 109 plaque-forming units/mL, providing virus quantities appropriate for in vivo animal studies. Additional quality control assays to ensure safety in vivo are described.
07:04
Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology
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Cited by 1 •
2025
This article presents a simplified method for preparing 4 hemagglutination units antigen for Newcastle disease virus serological testing. By accurately determining the hemagglutination titer of the antigen, combined with a more rigorous back-titration method, and a well-defined adjustment process, it enhances test efficiency, reduces false positives, and improves disease surveillance in poultry.
01:29
Respiratory Syncytial Virus Disease
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2026
Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...
10:14
Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease
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Cited by 6 •
2015
To investigate the blood-retinal barrier permeability and the inner limiting membrane integrity in animal models of retinal disease, we used several adeno-associated virus (AAV) variants as tools to label retinal neurons and glia. Virus mediated reporter gene expression is then used as an indicator of retinal barrier permeability.
FAQs
The two surface proteins act at different stages of entry. Hemagglutinin-neuraminidase recognizes and attaches to sialic acid receptors on susceptible bird cells, whereas the fusion protein enables the viral and cellular membranes to merge. Separating attachment from membrane fusion helps explain how NDV initiates infection and why both proteins matter when studying viral entry.
After entry, NDV replication remains in the cytoplasm rather than requiring the viral genome to enter the nucleus. Its RNA polymerase copies the negative-sense RNA genome and directs production of viral proteins. This linked copying-and-expression system is central to understanding how the virus generates the components needed for continued replication in infected cells.
Host range and viral evolution are important because NDV biology is not limited to a single question about replication. Studying which hosts the virus can infect, together with how viral populations change over time, places individual infections in a broader biological context. These themes also connect laboratory investigation with surveillance and interpretation of observed viral patterns.
Surveillance tracks the presence and distribution of NDV, while molecular diagnostics examines the virus through its biological and genetic features. The virus's RNA genome and defined replication machinery provide biological features relevant to investigation. Together, these approaches help identify viral activity and support monitoring of a pathogen that threatens poultry health and production.
Vaccine development draws on understanding the viral components and infection processes that need to be addressed. NDV research therefore incorporates knowledge of receptor attachment, membrane fusion, genome copying, and viral protein synthesis. Applying this biology to vaccine work can support efforts to protect poultry from Newcastle disease and reduce consequences for poultry health and production.
NDV provides a system for examining several broad virology questions at once, including paramyxovirus replication, host range, and viral evolution. Its defined entry proteins and cytoplasmic RNA replication also connect molecular mechanisms with larger biological patterns. For biology research, this makes NDV relevant not only to poultry disease but also to comparative studies of viral behavior.