Size Selection Filtration
Size Selection Filtration - Related Videos
12:47
Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
0 Views •
Cited by 20 •
2012
Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.
01:32
Filtration and Urine Formation
0 Views •
2019
The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys. Blood enters the renal...
13:26
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
0 Views •
Cited by 9 •
2015
This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.
01:26
Glomerular Filtration: Net Filtration Pressure
0 Views •
2025
Glomerular filtration, a key process in the kidneys, is regulated by three main pressures: Glomerular blood hydrostatic pressure (GBHP), Capsular hydrostatic pressure (CHP), and Blood colloid osmotic pressure (BCOP). GBHP, with an average value of 55 mmHg, promotes filtration by pushing water and solutes through the filtration membrane. This is balanced by two opposing forces: CHP, a "back pressure" exerted against the filtration membrane by fluid already in the capsular space and renal tubule,...
10:58
Isolation of Fecal Bacteria from Water Samples by Filtration
0 Views •
2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events include parasites, bacteria, and viruses that are shed in high numbers in the feces of infected people and animals. Transmission to new and susceptible hosts may...
FAQs
The pore size determines which sample components remain in the retained fraction and which enter the filtrate. Selecting a pore size in relation to the dimensions of the target cells, particles, or molecules can favor recovery of the desired fraction or removal of unwanted material. This choice directly influences sample purity and the suitability of the separated material for later analysis.
These forces provide different ways to move the fluid through the porous material. Gravity can support relatively straightforward flow, while pressure or centrifugation can drive separation when additional force is needed. The driving condition affects whether the sample passes through the filter efficiently, making it an important part of adapting the method to the biological material and desired outcome.
Removing unwanted components or isolating a more uniform size fraction reduces variation in the material used for subsequent work. Size selection filtration can therefore improve sample purity and make downstream results more consistent. This is particularly useful when differences in debris, cells, microorganisms, or other particles might otherwise complicate interpretation of biological analyses.
Planning should begin with the dimensions of the components to be separated and the pore size needed to distinguish them. Researchers must also select a suitable way to drive fluid flow, such as pressure, gravity, or centrifugation. These choices determine whether the target is concentrated, retained, or allowed to pass, and they shape the quality of the resulting sample.
In cell fractionation, the method helps separate cellular components according to their physical dimensions. It can also remove larger or smaller debris from a biological preparation, depending on the selected pore size and the fraction being collected. These uses produce a cleaner sample for downstream analysis without requiring the separation to rely on a molecular property.
Researchers can use size selection filtration to concentrate microorganisms or prepare samples containing a desired size range before analysis. The process may also remove unwanted material that would interfere with later measurements. Because the approach is relatively simple and scalable, it can support preparation of biological samples when consistent composition and improved purity are important.