Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

This video demonstrates the use of an automated gel size selection instrument to achieve improved reads in next generation sequencing platforms. First environmental water samples are collected and passed through a series of filters to systematically separate eukaryotic bacterial and viral sized particles. The retained bacteria are further concentrated by centrifugation.

Then the bacterial DNA is extracted. The extracted DNA is used to prepare libraries in which adapter and indexes are incorporated into fragmented DNA. The DNA is then amplified by PCR, and samples are loaded into the electrophoresis workstation.