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Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
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JoVE Journal Environment
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

DOI:
10.3791/52685-v

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13:26 min

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April 17, 2015

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, , , , , ,
1Department of Pathology and Laboratory Medicine, Faculty of Medicine,The University of British Columbia, 2Coastal Genomics, 3British Columbia Public Health Microbiology and Reference Laboratory

Chapters

  • 00:05Title
  • 02:40Water Collection and Filtration
  • 04:17Bacterial Concentration, DNA Extraction and Purification
  • 07:52DNA Library Preparation
  • 08:43Automated Gel Size Selection, Library Normalization, and Sequencing
  • 11:11Representative Analysis of Size Selected DNA Libraries
  • 12:51Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.

Tags

Keyword Extraction: Next-generation SequencingEnvironmental Water SamplesDNA Library PreparationGel Size SelectionAutomated ElectrophoresisRanger TechnologyRead LengthRead Quality

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