Superfusion Perfusion Modes
Superfusion Perfusion Modes - Related Videos
09:10
Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism
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Cited by 22 •
2019
Normothermic ex situ heart perfusion (ESHP), preserves the heart in a beating, semi-physiologic state. When performed in a working mode, ESHP provides the opportunity to perform sophisticated assessments of donor heart function and organ viability. Here, we describe our method for myocardial performance evaluation during ESHP.
09:24
Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
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Cited by 30 •
2011
This article describes the basic procedures for conducting optical mapping experiments in the Langendorff-perfused rabbit heart using the panoramic imaging system, and the dual (voltage and calcium) imaging modality.
01:07
What is a Mode?
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2023
The mode is one of the commonly used measures of a central tendency. It is defined as the most frequent value in a data set. There can be more than one mode in a data set if multiple values have the same highest frequency. For instance, suppose that the Statistics exam scores of 20 students are: 50; 53; 59; 59; 63; 63; 72; 72; 72; 72; 72; 76; 78; 81; 83; 84; 84; 84; 90; 93. Here, the mode is 72, as it occurs most frequently, five times. A data set with two modes is called bimodal. For example,...
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
01:14
Ventilatory Modes
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2025
Mechanical ventilators are life-saving devices that support or replace spontaneous breathing. They deliver breaths to patients through varying methods known as ventilator modes. Understanding these modes is critical for healthcare providers managing patients with respiratory failure. There are three ventilatory modes: full support, partial support, and spontaneous. These are described below. Full Support Modes Full support modes include controlled mechanical ventilation, continuous mandatory...
FAQs
Flow across a preparation and flow through it create different paths for solution movement and removal. These arrangements can change how quickly oxygenated medium reaches the biological material, how evenly a treatment contacts it, and how rapidly released substances leave the preparation. Selecting the direction therefore helps researchers control exposure and washout during dynamic experiments.
A pump or gravity-driven reservoir establishes the incoming solution flow and helps maintain defined chemical conditions around the preparation. The delivery approach influences how consistently medium arrives and how quickly applied substances are replaced or removed. Controlled flow is especially important when investigators compare responses across treatments or examine changes that develop over short time intervals.
Removing the bathing fluid prevents released metabolites, drugs, and signaling molecules from remaining indefinitely around the preparation. The effluent can therefore reflect substances produced or applied during the experiment while reducing continued exposure from the previous solution. This separation supports clearer interpretation of secretion, pharmacological responses, and other time-dependent biological changes.
An experiment begins by placing the isolated tissue, organ, or cell preparation in the chosen superfusion arrangement. Researchers then supply oxygenated physiological solution through a pump or reservoir, maintain the selected delivery pattern, and remove the outgoing fluid. They can introduce brief treatments and observe how the preparation responds as exposure and washout proceed.
They are useful when researchers need precise timing for solution changes or want to monitor rapid responses in a living preparation. Continuous replacement supports studies of tissue viability, secretion, electrophysiology, contractility, and pharmacological effects. The method is particularly relevant when a brief treatment must be distinguished from the preparation’s response during subsequent washout.
Depending on the preparation and readout, these experiments can track viability, secretory activity, electrical behavior, contractile responses, or reactions to pharmacological treatments. Because the incoming medium and effluent change over time, investigators can relate an observed response to treatment delivery and removal. This temporal control also improves reproducibility when comparing biological conditions.