Tiger Salamander Model
Tiger Salamander Model - Related Videos
10:07
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
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Cited by 12 •
2016
We present simple techniques for isolating giant transcriptionally active lampbrush chromosomes from living oocytes of frogs and salamanders. We describe how to observe these chromosomes "alive" by phase contrast or differential interference contrast, and how to fix them for fluorescent in situ hybridization or immunofluorescent staining.
12:10
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
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Cited by 7 •
2014
RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.
11:39
Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
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Cited by 29 •
2013
We describe the preparation of thin retinal slices from aquatic tiger salamanders (Ambystoma tigrinum) and explain how we use these slices to study synaptic processing in the retina by obtaining dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells.
19:33
Whole-cell Recordings of Light Evoked Excitatory Synaptic Currents in the Retinal Slice
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Cited by 2 •
2008
This video shows the process of whole-cell voltage clamp recordings in the retinal slice of the aquatic tiger salamander. We demonstrate the preparation of the slice as well as how to perform patch clamp recordings during visual stimulation of the retina.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
FAQs
After injury, salamanders can heal the wound and form a specialized blastema, a regenerative structure associated with renewed patterning. This sequence gives researchers a way to examine how cells and molecular signals may coordinate tissue replacement and restoration of body organization, making the model relevant to tissue repair research.
Renewed patterning helps organize regenerated tissue so that repair is linked to the body’s existing structure rather than treated as simple wound closure. Studying this process allows investigators to connect visible tissue recovery with underlying developmental mechanisms, supporting broader research on how organisms rebuild damaged parts.
External development permits researchers to observe growth and metamorphosis directly rather than relying only on changes hidden inside an adult organism. Eggs and aquatic larvae therefore provide a practical system for following developmental transitions and examining how controlled environmental conditions influence those observable stages.
Researchers can alter water chemistry under controlled conditions and observe resulting responses in aquatic larvae or developing animals. These comparisons help connect environmental changes with physiology and development, while also supporting toxicology and environmental biology studies concerned with how organisms respond to potentially harmful conditions.
A basic investigation begins by observing eggs or aquatic larvae as they grow, then tracking developmental changes through metamorphosis. Researchers can maintain controlled conditions, including selected water chemistry, and compare observations across conditions. The resulting records can show how development and physiological responses vary with the experimental environment.
An injury study follows the response from wound healing through blastema formation and renewed patterning. Researchers can examine how the damaged area changes over time and use those observations to investigate cellular and molecular mechanisms associated with regeneration. This approach connects visible recovery with the broader biology of tissue repair.
It is useful when researchers need to examine how controlled environmental changes affect development, physiology, or survival-related responses in aquatic stages. Water chemistry experiments can support toxicology investigations, while broader observations contribute to ecological monitoring and help relate organismal responses to questions about environmental health.
The model links organismal traits with both internal biological processes and external conditions. Development and regeneration reveal how salamanders organize growth and repair, whereas environmental studies show how those traits respond to changing surroundings. Together, these applications support biological questions about adaptation and provide context for assessing environmental health.