A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Surgery, Faculty of Medicine,University of Alberta, 2Department of Pediatrics, Faculty of Medicine,University of Alberta, 3Department of Biomedical Engineering, Faculty of Medicine,University of Alberta, 4Department of Mechanical Engineering, Faculty of Engineering,University of Alberta, 5Department of Chemical and Materials Engineering, Faculty of Engineering,University of Alberta, 6Canadian National Transplant Research Program, 7Department of Physiology, Faculty of Medicine,University of Alberta
Chapters
- 00:04Title
- 00:55Blood Collection and Heart Procurement
- 02:46Ex Situ Heart Perfusion (ESHP) Apparatus Placement and Perfusion Initiation
- 05:32Function Assessment and ESHP Metabolic Assessment
- 07:01Results: Representative ESHP Analyses
- 08:20Conclusion
Normothermic ex situ heart perfusion (ESHP), preserves the heart in a beating, semi-physiologic state. When performed in a working mode, ESHP provides the opportunity to perform sophisticated assessments of donor heart function and organ viability. Here, we describe our method for myocardial performance evaluation during ESHP.
Tags
Keywords:
Normothermic Ex Situ Heart PerfusionWorking ModeCardiac FunctionMetabolismDonor Heart PreservationHeart TransplantationFunctional EvaluationMetabolic AssessmentTechnical ExpertiseHeart RetrievalHeart ConnectionMedian SternotomyPericardiumVenous CannulaRight AtriumKrebs-Henseleit BufferAlbuminCardioplegiaAortic ClampIschemiaLeft Atrial OrificeInferior Vena Cava
