13,116 Views
•
09:10 min
•
January 12, 2019
DOI:
Summary
Automatically generated
Normothermic ex situ heart perfusion (ESHP), preserves the heart in a beating, semi-physiologic state. When performed in a working mode, ESHP provides the opportunity to perform sophisticated assessments of donor heart function and organ viability. Here, we describe our method for myocardial performance evaluation during ESHP.
Explore More Videos
Related Videos
11:38
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
Related Videos
26220 Views
08:30
Murine Isolated Heart Model of Myocardial Stunning Associated with Cardioplegic Arrest
Related Videos
11645 Views
06:26
Pre-clinical Model of Cardiac Donation after Circulatory Death
Related Videos
7886 Views
08:01
Semi-Minimal Invasive Method to Induce Myocardial Infarction in Rats and the Assessment of Cardiac Function by an Isolated Working Heart System
Related Videos
8086 Views
12:43
Rodent Working Heart Model for the Study of Myocardial Performance and Oxygen Consumption
Related Videos
20155 Views
12:24
Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart
Related Videos
14657 Views
08:21
Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography
Related Videos
2036 Views
09:04
Rat Model of Normothermic Ex-Situ Perfused Heterotopic Heart Transplantation
Related Videos
1030 Views
08:49
Cardiac Loading using Passive Left Atrial Pressurization and Passive Afterload for Graft Assessment
Related Videos
678 Views
![Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy](https://cloudfront.jove.com/CDNSource/teasers/63188.jpg)
14:56
Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
Related Videos
1395 Views
Read Article
Cite this Article
Hatami, S., White, C. W., Ondrus, M., Qi, X., Buchko, M., Himmat, S., Lin, L., Cameron, K., Nobes, D., Chung, H., Nagendran, J., Freed, D. H. Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (143), e58430, doi:10.3791/58430 (2019).
Copy