Normothermic ex situ heart perfusion (ESHP), preserves the heart in a beating, semi-physiologic state. When performed in a working mode, ESHP provides the opportunity to perform sophisticated assessments of donor heart function and organ viability. Here, we describe our method for myocardial performance evaluation during ESHP.
Hatami, S., White, C. W., Ondrus, M., Qi, X., Buchko, M., Himmat, S., Lin, L., Cameron, K., Nobes, D., Chung, H., Nagendran, J., Freed, D. H. Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (143), e58430, doi:10.3791/58430 (2019).