Subcutaneous Tissue
Subcutaneous Tissue - Related Videos
09:01
A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
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Cited by 7 •
2017
We standardized an abdominal adipose tissue biopsy using a non-diathermy method performed under local anesthesia. Three cases of excessive post-operation bleeding out of 115 operations (2.61%) occurred.We conclude that an abdominal adipose tissue surgical biopsy using a non-diathermy method can be safely applied to healthy men.
09:20
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Cited by 47 •
2016
This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.
08:59
Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique
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Cited by 5 •
2021
The manuscript and associated video demonstrate a percutaneous biopsy technique to obtain samples of subcutaneous adipose tissue from areas surrounding the umbilicus. This method is a low-risk and efficient way to investigate a range of parameters (e.g., gene or protein expression, enzyme activity, lipid content) within adipose tissue.
10:14
Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets
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Cited by 4 •
2020
In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.
09:35
Subcutaneous Trigeminal Nerve Field Stimulation for Refractory Facial Pain
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Cited by 8 •
2017
Treating chronic or neuropathic facial pain can be challenging when medical or standard treatment fails. Subcutaneous nerve field stimulation is the least invasive form of neuromodulation and is used for chronic back pain. We applied this technology to treat chronic and neuropathic trigeminal facial pain.
FAQs
The extracellular matrix provides a structural environment through which cells interact and exchange signals. In subcutaneous tissue, its relationship with adipose cells, immune cells, and blood vessels can shape nutrient exchange, inflammation, tissue repair, and tumor behavior. Studying these interactions helps researchers assess how the surrounding environment may support or alter tumor growth and responses to treatment.
Immune cells and blood vessels make this compartment biologically active rather than merely supportive. Immune cells can participate in local inflammatory signaling, while blood vessels contribute to nutrient exchange within the tissue. Examining both populations helps researchers evaluate how the local environment relates to tumor growth, invasion, treatment response, and possible movement of cancer cells.
Adipose cells contribute to the tissue’s energy-storage function and form part of the cellular environment surrounding a tumor. Their proximity to extracellular matrix, immune cells, and vessels gives researchers a way to examine how metabolic support and local signaling relate to cancer progression. This perspective broadens analysis beyond tumor cells alone and emphasizes interactions within the surrounding tissue.
Investigations can address several stages and patterns of cancer behavior, including tumor growth, local invasion, inflammation, and metastatic spread. Researchers can also examine how tumors interact with surrounding connective and adipose tissue and how those interactions affect treatment responses. Studying these processes in the same tissue context may clarify relationships that are not apparent when tumor cells are considered in isolation.
Cancer models that include subcutaneous tissue can help represent interactions between tumors and their surrounding microenvironment. Researchers may use these models to study cellular signaling, nutrient exchange, inflammation, tissue repair, and tumor responses to therapy. The resulting observations can improve understanding of cancer progression by incorporating the connective tissue, adipose, vascular, and immune components surrounding the tumor.
Because it can serve as a route for administering injectable therapies, subcutaneous tissue has practical importance in cancer research and treatment development. Researchers may consider how the local tissue environment relates to delivery and treatment response while also studying its biological role in tumors. This connects administration-related questions with broader investigations of inflammation, cellular interactions, and tumor progression.