Membrane Characterization

Membrane characterization is the systematic analysis of a membrane’s chemical composition, structure, physical properties, and performance. In chemistry, researchers combine methods such as spectroscopy, microscopy, thermal analysis, and permeability testing to relate molecular features, pore structure, surface chemistry, and mechanical behavior to membrane function. Measurements may reveal how solvents, solutes, ions, or gases interact with and pass through the material under defined conditions. These data support membrane design and quality assessment for separation science, catalysis, energy storage, water purification, and biomedical applications, while helping researchers optimize selectivity, stability, transport rates, and resistance to fouling.

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JoVE Journal - Engineering

Fabrication and Characterization of High-Q Silicon Nitride Membrane Resonators

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Cited by 1 •

2025

This article traces the life cycle of a silicon nitride membrane resonator, from design through fabrication to characterization.

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

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Cited by 4 •

2019

We describe a method for the characterization of proton-driven membrane transporters in membrane vesicle preparations produced by heterologous expression in E. coli and lysis of cells using a French press.

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

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Cited by 4 •

2015

The first part of this article shows how to select mutant cell lines expressing vesicular Na+/H+ exchangers at their plasma membrane. The second part provides protocols based on intracellular pH measurements and fast ion uptake, which are used to determine the ion selectivity and the kinetic parameters of these exchangers.

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

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Cited by 3 •

2014

Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

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Cited by 9 •

2018

We report methods for characterization of MLKL-mediated plasma membrane rupture in necroptosis including conventional and confocal live-cell microscopy imaging, scanning electron microscopy, and NMR-based lipid binding.

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