Single Terminated Surfaces
Single Terminated Surfaces - Related Videos
09:16
Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
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Cited by 12 •
2012
Here we describe a protocol for the preparation of agar-embedded retinal slices that are suitable for electrophysiology and Ca2+ imaging. This method allows one to study ribbon-type synapses in retinal microcircuits using direct patch-clamp recordings of single presynaptic nerve terminals.
01:44
Termination of Translation
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2020
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
10:35
Surface Passivation for Single-molecule Protein Studies
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Cited by 270 •
2014
We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.
06:45
In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal
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Cited by 14 •
2018
Here we illustrate how single molecule photo-activated localization microscopy can be carried out on the motor nerve terminal of a live Drosophila melanogaster third instar larva.
15:16
Loading Drosophila Nerve Terminals with Calcium Indicators
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Cited by 17 •
2007
Calcium is a ubiquitous messenger in the nervous system, essential for triggering neurotransmitter release and changes in synaptic strength. Here we demonstrate a technique for loading Ca2+-indicators into Drosophila nerve terminals. We also demonstrate fabrication of the required apparatus and emphasize points critical for the technique's success.
FAQs
Satisfying dangling bonds reduces the number of highly reactive, unsatisfied surface sites and establishes a more consistent chemical environment at the interface. That change can alter surface energy, electronic structure, wettability, and chemical stability. Consequently, the same underlying solid may show different adsorption behavior or interfacial reactivity after its outermost bonding environment is controlled.
A dominant surface species presents a more chemically consistent set of sites to incoming molecules or adjacent materials. This uniformity can make adsorption and interfacial reactions more predictable than on a compositionally varied surface, where different sites may behave differently. In chemistry research, that predictability helps connect atomic-scale surface composition with measured reaction or compatibility outcomes.
The key distinction is the degree of chemical uniformity at the outermost layer. A single dominant termination offers a narrower range of surface environments, whereas mixed composition can produce multiple types of reactive or weakly interacting sites. This difference affects how scientists interpret surface energy, wettability, adsorption, and stability, especially when relating measurements to atomic-scale structure.
Evaluation should focus on properties directly linked to the outermost chemical layer: surface energy, electronic structure, wettability, and chemical stability. Researchers can then consider how those characteristics influence adsorption, interfacial reactions, or interactions with deposited films and biological molecules. Examining these connections shows whether the selected termination produces the intended functional interface.
They are useful when researchers need to tune how a solid interacts with its surroundings rather than simply characterize the bulk material. Applications identified for controlled termination include catalysts, sensors, electronic materials, and other functional interfaces. The approach is valuable because changes at the surface can regulate adsorption, reactions, corrosion resistance, and compatibility with neighboring materials.
The outermost chemical environment can determine whether an interface is compatible with a deposited film or with biological molecules. Changes in wettability, chemical stability, and surface reactivity may influence how those materials interact with the solid. Studying this relationship helps researchers design interfaces with more suitable behavior for electronic, materials, or bio-related applications.