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In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal
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JoVE Journal Neuroscience
In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

DOI:
10.3791/56952-v

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06:45 min

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January 14, 2018

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, , , , ,
1Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute,The University of Queensland, 2VIB Centre for Brain and Disease Research, KU Leuven Department of Neurosciences,Leuven Institute for Neurodegenerative Disease (LIND), 3Queensland Brain Institute,The University of Queensland

Chapters

  • 00:00Title
  • 00:29Design of transgenic Drosophila
  • 00:44Dissection of third-instar Drosophila larva
  • 02:31Single particle tracking photo-activated localization microscopy
  • 04:31Data analysis
  • 05:13Representative results
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

Here we illustrate how single molecule photo-activated localization microscopy can be carried out on the motor nerve terminal of a live Drosophila melanogaster third instar larva.

Tags

Keywords: Single-molecule TrackingDrosophilaPresynaptic Motor Nerve TerminalIn VivoPhotoconvertible FluorophoreSchneider’s Insect MediumDissectionSylgard BaseTIRF MicroscopyNeuromuscular Junctions

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