E-cadherin Immunodetection
E-cadherin Immunodetection - Related Videos
01:25
Structure of Cadherins
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2023
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
07:11
A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
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Cited by 5 •
2017
The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).
01:19
Cadherins in Tissue Organization
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2023
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues. Cell Sorting During Development Cell sorting plays an...
11:53
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
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Cited by 8 •
2014
Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.
04:42
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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2016
Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.
FAQs
Because E-cadherin is calcium-dependent, its detected pattern provides a molecular readout connected to cell-cell adhesion rather than merely a general marker of tissue structure. The observed location shows where the protein is present, while signal abundance can indicate relative differences between samples. This distinction helps relate adhesion-associated changes to epithelial organization during developmental remodeling.
Distribution and abundance answer different developmental questions. Distribution indicates how E-cadherin is positioned within the examined tissue or cells, whereas abundance indicates how much signal is detected. Considering both measurements can distinguish a redistribution of the protein from an overall change in its detected level, improving interpretation of epithelial integrity, tissue remodeling, and epithelial-to-mesenchymal change.
Changes in E-cadherin staining can connect cell-adhesion behavior with larger developmental outcomes. During morphogenesis, researchers can examine whether epithelial organization and tissue remodeling coincide with altered protein location or abundance. Extending the comparison across developmental stages or experimental conditions helps reveal how adhesion-associated changes relate to organ formation, while also providing context for epithelial-to-mesenchymal transitions.
After tissue or cells are fixed, the sample is typically permeabilized so antibody reagents can be applied to the specimen. A primary antibody directed against E-cadherin is added first, followed by a labeled secondary antibody. The resulting fluorescence or enzyme-generated signal is then examined to determine the protein’s location and relative abundance under the selected experimental conditions.
Both signal formats make the antibody-bound target observable, but they provide different readout formats for the same detection strategy. Fluorescence can display staining patterns directly as an optical signal, whereas an enzyme-generated signal produces a detectable reaction product. In either case, interpretation focuses on where E-cadherin appears and how its detected abundance differs across samples.
Such comparisons are useful when development involves changing epithelial organization, tissue remodeling, or organ formation. Examining staining across developmental stages can show how E-cadherin distribution and abundance change over time, while comparisons between experimental conditions can associate altered patterns with specific developmental contexts. The results can also help assess whether an epithelial-to-mesenchymal change accompanies those tissue-level events.