Stirred Suspension
Stirred Suspension - Related Videos
10:20
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
0 Views •
Cited by 13 •
2017
This video and manuscript describe an emulsion-based method to encapsulate mammalian cells in 0.5% to 10% alginate beads which can be produced in large batches using a simple stirred vessel. The encapsulated cells can be cultured in vitro or transplanted for cellular therapy applications.
10:17
The Tail Suspension Test
0 Views •
Cited by 534 •
2012
The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test.
10:49
Power Input Measurements in Stirred Bioreactors at Laboratory Scale
0 Views •
Cited by 9 •
2018
The power input in stirred bioreactors can be measured through the torque that acts on the impeller shaft during rotation. This manuscript describes how an air bearing can be used to effectively reduce friction losses observed in mechanical seals and improve the accuracy of power input measurements in small-scale vessels.
04:16
ECM Suspension Preparation: A Technique to Obtain Homogenous Suspension from Tissue-derived Extracellular Matrix
0 Views •
2023
This video describes the preparation of a homogenized and digested extracellular matrix suspension from decellularized and lyophilized tissues. This technique helps in fabricating ECM-derived porous foams and microcarriers for use as substrates in advanced 3D cell culture models.
01:17
Colloids and Suspensions
0 Views •
2023
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
FAQs
Stirring regulates aggregate size by combining fluid movement with shear forces. These forces can influence how readily cells or multicellular groups remain together, break apart, or develop into differently sized structures. Because aggregate dimensions affect the culture environment, adjusting agitation provides a way to improve uniformity while also testing how mechanical conditions shape developmental behavior.
Agitation helps distribute nutrients and oxygen more evenly throughout the liquid medium, reducing differences between locations within the culture. More uniform environmental conditions can support greater consistency among developing aggregates and make observed developmental differences easier to interpret. This circulation is especially relevant when three-dimensional structures grow with cells positioned at varying distances from the surrounding medium.
Shear forces generated by fluid movement provide a physical influence in addition to the cells’ intrinsic developmental programs. By changing stirring conditions, researchers can examine how mechanical forces affect aggregate formation, size, and organization. This makes the culture useful for studying the interaction between environmental physics and processes such as differentiation or morphogenesis.
Compared with surface-dependent culture, this approach allows developmental systems to form and persist as suspended three-dimensional groups. That distinction matters because organization occurs within aggregates rather than being constrained primarily by attachment to a solid surface. The resulting setup is useful for examining tissue organization and morphogenesis in a three-dimensional context.
A basic workflow begins by placing cells or multicellular aggregates in liquid culture medium, then maintaining continuous agitation so the material remains distributed through the culture. Researchers can adjust stirring conditions during growth to influence aggregate size and uniformity. This controlled setup supports three-dimensional developmental cultures for studying cell differentiation and tissue organization.
Developmental biologists apply Stirred Suspension to embryoid bodies, organoid-forming aggregates, and related three-dimensional cell systems. The method provides a setting in which cells can differentiate while participating in collective tissue organization. It is therefore useful for investigating how developmental programs produce organized structures and morphogenetic changes under controlled culture conditions.
Observed differences in culture outcome can be considered alongside stirring conditions rather than attributed only to cellular programs. Changes in aggregate size, culture uniformity, or developmental organization may reflect altered fluid movement, shear forces, nutrient distribution, or oxygen availability. This makes the system valuable for linking physical culture conditions with developmental biology outcomes.