Porcine Skin Processing
Porcine Skin Processing - Related Videos
08:31
Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
0 Views •
Cited by 14 •
2017
This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.
14:14
Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics
0 Views •
Cited by 9 •
2017
This protocol uses multi-view stereo to generate three-dimensional (3D) models out of uncalibrated sequences of photographs, making it affordable and adjustable to a surgical setting. Strain maps between the 3D models are quantified with spline-based isogeometric kinematics, which facilitate representation of smooth surfaces over coarse meshes sharing the same parameterization.
03:37
Porcine Heart Excision: A Protocol to Extract Porcine Heart for Cardiac Transplant
0 Views •
2025
In this video, we describe a step-by-step protocol to extract the heart from a porcine model for cardiac transplantation.
05:07
Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
0 Views •
Cited by 20 •
2018
The practical application and performance of methods for generation of representative tissue samples of porcine animal models for a broad spectrum of downstream analyses in biobank projects are demonstrated, including volumetry, systematic random sampling, and differential processing of tissue samples for qualitative and quantitative morphologic and molecular analyses types.
03:02
Porcine Cumulus Oocyte Complex Isolation: A Technique to Isolate Cumulus Oocyte Complex from Porcine Ovarian Follicle for In Vitro Fertilization
0 Views •
2025
This video describes the technique to isolate the cumulus-oocyte complex or COC from porcine ovaries. The isolated intact COC can be used to carry out the in vitro fertilization - the assisted reproductive technique for developing fertility treatments.
FAQs
The collagen-rich extracellular matrix provides the structural basis for the processed tissue, so removing cellular components must be balanced with matrix preservation. This balance allows the material to retain skin-like structural characteristics while becoming suitable for engineering studies. Maintaining that matrix helps researchers evaluate scaffold behavior, tissue responses, and biomaterial performance without relying solely on intact cellular tissue.
Processing can modify several properties relevant to engineering experiments, including tissue thickness, sterility, and mechanical behavior. These variables determine how consistently the material can be handled and compared across studies. Controlling them is especially important when porcine skin is used as a scaffold, tissue model, or test material for evaluating biological responses and biomaterial performance.
Chemical and enzymatic treatments provide alternative ways to remove cellular components from the tissue while retaining its collagen-rich extracellular matrix. Their role is not simply to clean the surface, but to prepare a matrix-based material for subsequent research use. Selecting and controlling the treatment approach helps produce tissue with properties appropriate for the intended model or biomaterials experiment.
Standardized processing reduces variation in preparation by controlling cleaning, trimming, cellular-component removal, thickness, sterility, and mechanical properties. More consistent materials make it easier to compare scaffold performance and biological responses between experiments. In engineering research, this reproducibility supports more reliable evaluation of wound-healing models, tissue-engineering strategies, drug-delivery systems, and surgical approaches.
A typical workflow begins with cleaning and trimming the skin, followed by chemical or enzymatic treatment to remove cellular components. Further processing can adjust thickness, sterility, and mechanical properties before the material enters an experiment. Each stage prepares the tissue for a defined engineering use while helping maintain the collagen-rich extracellular matrix required for skin-like modeling.
Researchers may select the material when they need a skin-like scaffold or tissue model for wound-healing studies, tissue engineering, drug delivery, or surgical research. Its structural and functional similarities to human skin provide relevant experimental context, while controlled processing supports reproducible testing. The resulting data can help assess biomaterial performance and biological responses in engineering studies.