Petri Dish Modification

Petri dish modification is the deliberate alteration of a Petri dish or its internal conditions to support a specific laboratory investigation. Researchers may change the dish surface, add barriers or compartments, or control factors such as moisture, nutrients, temperature, and gas exchange, thereby shaping how samples interact with their surroundings. In environmental research, these modifications help model simplified habitats and examine microbial growth, contamination, biodegradation, or responses to environmental stress. By improving experimental control and reproducibility, modified dishes connect observations made in the laboratory with broader questions about ecosystems, environmental change, and the behavior of organisms under defined conditions.

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JoVE Core - Molecular Biology

Histone Modification

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2020

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression. Acetylation The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

Research

JoVE Journal - Immunology and Infection

Microtiter Dish Biofilm Formation Assay

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Cited by 2243 •

2011

The assay describes a rapid means to measure early biofilm formation in bacteria and fungi. This method uses a microtiter plate as the substratum for microbial biofilm formation, and the biofilm is visualized using crystal violet strain. The assay provides either a qualitative or quantitative assay for early biofilm formation.

Spreading of Chromatin Modifications

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2020

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex. Writers The writer is an enzyme that can...

Research

JoVE Journal - Biology
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Detection of Histone Modifications in Plant Leaves

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Cited by 22 •

2011

A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.

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