Fluorescent Probe Detection
Fluorescent Probe Detection - Related Videos
06:58
Synthesis of an In vivo MRI-detectable Apoptosis Probe
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Cited by 3 •
2012
Early detection of apoptosis may identify at-risk cell populations in a variety of diseases. Here we demonstrate a method to link an early apoptosis-detection protein (Annexin V) to a MRI-detectable iron oxide nanoparticle (SPIO). This method may be extended to other proteins of interest to generate MRI-detectable molecular imaging probes.
03:44
Detection of Inflammasome Activation via Fluorescence Microscopy
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2025
This video demonstrates a method to detect NLRP3 inflammasome activation in macrophages using fluorescent reporter probes. The cells are treated with potassium ionophores and glycine to induce inflammasome assembly and caspase-1 activation. Fluorescent reporter probes visualize activated caspase-1, and cells are stained with a fluorescent dye for nuclei visualization.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
10:59
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
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Cited by 9 •
2012
A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition.
04:02
An Assay to Measure the Accumulation of a Fluorescent Antibiotic Probe in Bacterial Cells
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2025
This video demonstrates an assay to measure the accumulation of a fluorescent antibiotic probe in bacterial cells. Exposure to an efflux pump inhibitor disrupts the transmembrane proton gradient in bacterial cells, upon which a fluorophore-conjugated antibiotic is added. Inhibition of efflux pumps due to the disrupted proton gradient causes accumulation of the antibiotic inside the bacteria. Bacterial cells are lysed, and the fluorescence intensity is measured to confirm antibiotic accumulation.
FAQs
A target interaction or chemical reaction alters a probe’s fluorescence, which can be observed as a change in intensity, wavelength, or lifetime. These signal dimensions provide different optical readouts of the same event. Measuring the response with suitable fluorescence equipment allows investigators to determine whether a target is present and assess changes in its environmental sample.
Each measurement describes a different feature of the probe’s optical response. Intensity indicates how strongly fluorescence changes, wavelength identifies a shift in the emitted signal, and lifetime records how long the fluorescence persists. Considering these properties helps characterize target-related changes and supports more informative analysis than relying on a single optical measurement.
Environmental conditions can influence chemical transport and biological activity, so the fluorescence response must be interpreted in relation to the sample context. Measurements from water, soil, and air may reflect different transport conditions or levels of ecosystem activity. Connecting probe signals with those conditions helps researchers study pollutant behavior and environmental health.
The workflow begins with selecting a molecular probe that responds to the chemical or biological target of interest, followed by examining the probe response in the environmental sample. Investigators then measure changes in fluorescence intensity, wavelength, or lifetime using a fluorometer or fluorescence microscope. The resulting optical signal supports target detection, screening, or monitoring.
A fluorometer is appropriate when the main goal is to measure the optical response from an environmental sample, while a fluorescence microscope is useful when spatial resolution is important. This distinction matters when researchers need either a signal measurement for screening and monitoring or information about where fluorescence occurs within a sample or biological context.
In environmental sciences, the method can be applied to water, soil, and air samples to identify contaminants, monitor pollutants, track metal ions, and assess biological activity. Its sensitivity supports rapid screening and process monitoring, while spatial resolution enables studies of how targets and environmental conditions relate to chemical transport and ecosystem health.