Cardiac Specific Promoter
Cardiac Specific Promoter - Related Videos
10:58
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
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Cited by 15 •
2017
Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.
08:52
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
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Cited by 13 •
2015
This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.
05:04
In vitro Assessment of Cardiac Reprogramming by Measuring Cardiac Specific Calcium Flux with a GCaMP3 Reporter
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Cited by 4 •
2022
We describe here, the establishment and application of an Tg(Myh6-cre)1Jmk/J /Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J (referred to as αMHC-Cre/Rosa26A-Flox-Stop-Flox-GCaMP3 below) mouse reporter line for cardiac reprogramming assessment. Neonatal cardiac fibroblasts (NCFs) isolated from the mouse strain are converted into induced cardiomyocytes (iCMs), allowing for convenient and efficient evaluation of reprogramming efficiency and functional maturation of iCMs via calcium (Ca2+) flux.
03:11
Recombinant Adeno Associated Virus Based Gene Delivery: A Technique for Transgene Delivery and Cardiac-Specific Expression in Rodent Model
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2025
In this video, we demonstrate recombinant adeno associated virus-based gene delivery in the mouse pup. This method helps in studying gene expression in the targeted cells.
02:40
The Eukaryotic Promoter Region
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2020
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
FAQs
Activation depends on cardiac-enriched transcription factors recognizing promoter elements and recruiting the transcriptional machinery. Binding connects the DNA sequence to RNA synthesis, but only when the relevant factors and cellular context are present. This mechanism explains why the same regulatory sequence can behave differently across tissues or developmental stages.
Activity is shaped by cell state, species, and experimental conditions. A sequence that appears selective in one heart-related model may show a different expression pattern in another because transcription-factor availability and developmental context can change. Researchers therefore interpret cardiac enrichment as context-dependent performance, not an invariant property of the DNA sequence.
No. Cardiac selectivity means expression is favored in heart muscle cells, while activity in other cell types is limited rather than necessarily eliminated. This distinction matters when interpreting experimental results, because residual expression outside cardiac tissue can affect how researchers assess promoter performance and the specificity of a genetic intervention.
Researchers place the regulatory sequence in front of a gene whose activity they want to favor in cardiac tissue. The promoter can then guide expression of a reporter, therapeutic transgene, or gene-editing component. Researchers evaluate the resulting pattern in the relevant cellular or developmental context to determine whether cardiac enrichment is achieved.
Reporter genes provide a visible or measurable readout of promoter activity in experimental systems. When directed by cardiac regulatory control, they help researchers examine where and when gene expression occurs in relation to heart muscle cells, development, or changing cellular states. This makes promoter behavior easier to study before applying the system to functional interventions.
This approach is useful when a therapeutic transgene or gene-editing component should act preferentially in cardiac tissue rather than broadly across cell types. Restricting expression can align the genetic intervention with the intended tissue context. Its suitability still depends on promoter behavior under the relevant species, cell state, developmental stage, and experimental conditions.
They provide a way to connect gene activity with heart development, cardiac function, and disease modeling. Researchers can use promoter-controlled reporters to track cardiac expression, or direct functional transgenes and editing components toward heart muscle cells. These applications help test genetic mechanisms while accounting for changes in promoter activity across biological contexts.