Kidney Serum Microrna
Kidney Serum Microrna - Related Videos
12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
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Cited by 11 •
2013
This article describes an in situ hybridization protocol optimized for colormetric detection of microRNA expression in formalin fixed kidney sections.
01:22
MicroRNAs
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2019
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
07:01
A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
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Cited by 1 •
2020
This article presents a protocol for detecting microRNA expression in the kidneys of an acute kidney injury mouse model using quantitative real-time reverse-transcription polymerase chain reaction. This protocol emphasizes an ischemic kidney injury mouse model and the careful extraction of microRNA samples.
09:05
MicroRNA-based Regulation of Picornavirus Tropism
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Cited by 4 •
2017
We describe here a method for regulating picornavirus tropism by incorporating sequences complementary to specific microRNAs into the viral genome. This protocol can be adapted to all different classes of viruses with modifications based upon the length and nature of their life cycle.
05:39
Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
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Cited by 2 •
2020
microRNAs are involved in the pathogenesis of IgA nephropathy. We have developed a reliable method for detecting microRNA expression levels in the kidneys of an IgA nephropathy mouse model (HIGA mice). This new method will facilitate to check for miRNAs involvement in IgA nephropathy.
FAQs
Kidney serum microRNAs influence gene output after entering regulatory interactions with messenger RNAs. By binding complementary sequences, they can reduce translation, the process that produces protein from mRNA, or promote mRNA degradation. This post-transcriptional control connects altered microRNA abundance with changes in cellular activity in kidney tissue.
Protection of circulating molecules by extracellular vesicles or protein complexes can help preserve them in serum. This stability matters because measured abundance may reflect molecules that remain detectable outside cells rather than only short-lived intracellular RNA. Protected forms therefore support interpretation of serum-based measurements in kidney research.
Because they are noncoding RNAs, kidney serum microRNAs illustrate how gene regulation can occur without encoding proteins. Their activity affects messenger RNA translation or stability, linking regulatory RNA patterns to cellular behavior. Studying these patterns adds a genetics-focused perspective on kidney development, injury, inflammation, and disease.
Reverse transcription quantitative PCR and sequencing provide complementary ways to measure microRNA abundance. Reverse transcription quantitative PCR supports targeted analysis of selected molecules, whereas sequencing can examine broader microRNA patterns. The choice depends on whether a study prioritizes focused measurement or wider profiling across kidney-related serum samples.
Serum-based measurement offers a minimally invasive way to investigate kidney-associated molecular changes. When microRNA abundance changes with kidney development, injury, inflammation, or disease, researchers can evaluate whether those patterns have value for diagnosis, prognosis, treatment-response assessment, or personalized renal-care strategies. This approach connects genetic regulation with clinically relevant monitoring.
Interpretation depends on the measured pattern and the research question, not simply on detecting a molecule. Increased or decreased abundance can be examined as an indicator associated with kidney development, injury, inflammation, or disease. Researchers may also assess whether the signal has potential relevance for prognosis, treatment response, diagnosis, or personalized approaches to renal disease.