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MicroRNA-based Regulation of Picornavirus Tropism
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Immunology and Infection
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JoVE Journal Immunology and Infection
MicroRNA-based Regulation of Picornavirus Tropism

MicroRNA-based Regulation of Picornavirus Tropism

DOI:
10.3791/55033-v

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09:05 min

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February 06, 2017

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,
1Department of Molecular Medicine,Mayo Clinic College of Medicine

Chapters

  • 00:05Title
  • 01:04Titrating Virus Stocks by Calculating 50% Tissue-culture Infectious Dose (TCID50)
  • 02:33Evaluating MicroRNA-targeting Efficacy: Single-step Growth Kinetics
  • 04:29Evaluating MicroRNA-targeting Specificity: Virus-spreading Assay Using Synthetic MicroRNA Mimics
  • 07:01Results: Comparing MicroRNA-targeted Virus to Unmodified Virus
  • 08:32Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a method for regulating picornavirus tropism by incorporating sequences complementary to specific microRNAs into the viral genome. This protocol can be adapted to all different classes of viruses with modifications based upon the length and nature of their life cycle.

Tags

Keywords: MicroRNAPicornavirusTropismVirus TargetingVirus SafetyVirus ManufacturingVirus UtilityVirus InfectionVirus ReplicationVirus PropagationVirus ToxicityVirus TiterCell InfectionCytopathic Effects96-well Plate12-well PlateSerum-free MediaComplete Media

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