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Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
JoVE Journal
Genetics
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JoVE Journal Genetics
Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

DOI:
10.3791/63258-v

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06:48 min

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April 29, 2022

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, , , , ,
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

Chapters

  • 00:04Introduction
  • 00:39Serum Sample Collection
  • 01:37Total RNA Extraction from Serum and cDNA Synthesis
  • 03:33qRT-PCR of miRNA
  • 05:27Results: Differentially Expressed miRNAs in the Kidneys and Serum of SAMP1 Mice
  • 06:15Conclusion

Summary

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We present a method for evaluating microRNA expression in the kidney and serum of mice with age-dependent renal impairment by quantitative reverse-transcription polymerase chain reaction.

Tags

Keywords: QRT-PCRMicroRNA ExpressionSerumAge-dependent Renal ImpairmentRNA ExtractionRNA IsolationReal-time PCRGene Expression Profiling

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