Anti-cd40 Stimulation
Anti-cd40 Stimulation - Related Videos
12:24
Murine Model of CD40-activation of B cells
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Cited by 13 •
2010
In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.
13:27
Generation of Human CD40-activated B cells
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Cited by 36 •
2009
In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.
11:02
Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network
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Cited by 17 •
2010
In this article, we examine the methodology and considerations relevant to the combination of TMS and fMRI to examine the effects of brain stimulation on the default network.
04:21
Generating Anti-Angiogenic Tumor-Associated Neutrophils
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2025
This video demonstrates an assay to generate anti-angiogenic tumor-associated neutrophils (TANs). Upon taking a murine melanoma tumor containing pro-angiogenic TANs, the single cells are isolated using fluorescence-activated cell sorting (FACS) and treated with an inhibitor of nicotinamide phosphoribosyl transferase (NAMPT) to reduce the capacity to stimulate angiogenesis.
10:15
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
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Cited by 46 •
2015
Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo.
FAQs
Agonistic anti-CD40 antibodies engage CD40 in place of CD40L, allowing researchers to isolate the consequences of this receptor pathway experimentally. CD40 engagement promotes signaling in CD40-expressing cells, including increased costimulatory molecule expression, cytokine production, and functional maturation. This makes the method useful for examining how CD40-dependent communication coordinates antigen presentation and downstream T-cell responses.
Dendritic cells and macrophages are prominent targets because their maturation and antigen-presenting functions respond to CD40 engagement. Examining these cells can reveal whether stimulation increases costimulatory molecule expression, alters cytokine production, or changes functional readiness to communicate with T cells. The same framework can also be applied more broadly to other immune cells that express CD40.
These readouts connect receptor engagement with immune function rather than merely confirming antibody binding. Increased costimulatory molecule expression can indicate improved capacity for antigen presentation and T-cell activation, while cytokine production reflects changes in immune coordination. Together with measures of cellular maturation, they help define how CD40 signaling shapes the quality of an immune response.
A study generally begins by selecting CD40-expressing immune cells, exposing them to an agonistic anti-CD40 antibody, and then evaluating relevant functional changes. Researchers can examine costimulatory molecule expression, cytokine production, and maturation of antigen-presenting cells. Linking these measurements to T-cell activation or antimicrobial responses helps determine the broader consequence of the induced CD40-dependent signaling.
Anti-CD40 stimulation can be used to investigate whether strengthening CD40-dependent communication improves antigen-presenting cell function and supports T-cell activation. In vaccine research, it helps evaluate CD40-related adjuvant activity. In immunotherapy studies, the approach provides an experimental way to examine how enhanced immune coordination may influence activation and the development of immune memory.
In infection research, the method helps separate CD40-dependent immune coordination from other signals generated during host-pathogen interactions. Researchers can assess effects on antigen-presenting cells, T-cell activation, antimicrobial responses, and immune memory. These outcomes clarify how communication through CD40 may contribute to protective immunity or shape the response to an infectious challenge.