Murine Model of CD40-activation of B cells

Hi, my name is Tanya Wig and I'm a PhD student and working in the laboratory for tumor immunology and transplantation immunology at the University Hospital of Cologne. My studies mainly focus on marine CD 40 activated B cells and their ability to induce immune responses in vivo. Originally, human CD 40 B cells were generated as alternative antigen presenting cells to dendritic cells.

Those cells are aimed to be used for immunotherapeutic purposes In clinical settings, CD 40 B cells are generated under CD 40 signaling and stimulation with IL four. According to this system, they can be expanded over several weeks. After optimizing the generation of human CD 40 B cells from PBMC in our lab, we transferred this principle successfully to mice and this is what I want to show you today.