Murine Model of CD40-activation of B cells

Journal

Immunology and Infection

JoVE Journal
Immunology and Infection
Author Produced

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JoVE Journal Immunology and Infection

Murine Model of CD40-activation of B cells

DOI:

10.3791/1734-v

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12:24 min

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March 05, 2010

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Tanja M. Liebig, Anne Fiedler, Nela Klein-Gonzalez, Alexander Shimabukuro-Vornhagen, Michael von Bergwelt-Baildon

¹Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine,University Hospital of Cologne

Chapters

00:00Title
00:14Introduction
02:10Preparation of Murine Splenocytes for CD40 – Stimulation
04:19Preparation of Feeder Cells
07:59CD40 – Stimulation
08:50Subcultivation
11:25Analysis of Murine CD40 – Activated B Cells
11:47Conclusion

Summary

Automatic Translation

In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.

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Chapters

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