B Cell Isolation
B Cell Isolation - Related Videos
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
04:32
Isolation of Mouse Peritoneal Cavity Cells
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Cited by 445 •
2010
The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.
11:52
Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
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Cited by 1 •
2017
We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.
13:42
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
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Cited by 3 •
2013
The presented method offers a way to detect functional effective cardiotropic autoantibodies in the plasma of patients with dilated cardiomyopathy, irrespective of the specific antigen, by analysing the impact of isolated patient immunoglobulin on cellular shortening and intracellular calcium transients in isolated rat cardiomyocytes.
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Cited by 151 •
2013
Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.
FAQs
Antibodies recognize surface markers expressed on B cells and connect those cells to magnetic beads or a flow-based sorting system. The instrument or magnetic field then separates labeled cells from the mixed population. Marker choice determines which B-cell population is captured, while the separation format influences how cells are recovered for later analysis or culture.
Isolation can be designed either to selectively retain labeled B cells or to label unwanted cells for removal. Retention directly enriches the population recognized by the chosen marker, whereas depletion leaves B cells behind after non-target cells are removed. This distinction affects the composition of the final sample and should match the intended downstream assay.
A density-gradient step can first reduce the complexity of a blood or tissue sample by separating cells according to density. Subsequent antibody-based enrichment then operates on a less mixed population, supporting more focused separation of B cells. Used as a preliminary stage, it can organize the workflow before magnetic or flow-based selection is performed.
Two important outcomes are purity and viability. Purity indicates how effectively unwanted cells were excluded, while viability reflects whether the recovered B cells remain suitable for continued analysis or culture. Antibody marker selection, separation strategy, and sample preparation all influence the resulting population and therefore the reliability of flow cytometry, sequencing, or functional assays.
A typical workflow begins with a blood, tissue, or mixed-cell sample, followed when appropriate by density-gradient separation. Researchers then apply antibodies against selected B-cell markers and use magnetic-bead enrichment or flow-based sorting to recover the target population. The isolated cells can subsequently be assessed for purity and viability before culture or downstream measurement.
Isolated B cells provide a focused system for examining antigen recognition, antibody production, activation, and differentiation. In infection studies, they can also support analysis of responses to pathogens without the complexity of the original mixed-cell sample. Their enrichment is particularly useful when downstream work includes flow cytometry, culture, sequencing, or functional assays.