Cascade Impactor
Cascade Impactor - Related Videos
03:24
Generating a Mouse Model of Traumatic Brain Injury Using an Impactor Device
0 Views •
2025
This video demonstrates the procedure for inducing traumatic brain injury (TBI) in a mouse model. An anesthetized mouse with an exposed skull is taken to create an opening in the skull, and then a controlled cortical injury is induced using an impact device. The injury damages the brain tissue, causing impaired cognitive function and motor coordination.
03:58
Generating a Traumatic Brain Injury Mouse Model Using an Impactor Probe
0 Views •
2025
This video demonstrates the procedure for inducing an open-skull traumatic brain injury in a mouse by making a midline skull incision, drilling through the skull and removing the bone flap to expose the brain cortex, and applying high pressure to induce trauma. The wound is then sutured to prepare the mouse model for subsequent experiments.
07:28
A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor
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Cited by 46 •
2012
A reliable and repeatable way to produce a cervical unilateral spinal cord injury using the Infinite Horizon impactor is described. The method takes advantage of a custom designed frame and clamp to stabilize the spine. The standardized procedure and biomechanical injury parameters result in sufficient and sustained injuries.
01:25
Rab Cascades
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2023
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
01:43
Intracellular Signaling Cascades
0 Views •
2021
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
FAQs
Particle inertia determines whether airflow can redirect the particle around a nozzle. As airflow accelerates through progressively smaller nozzles, particles with sufficient inertia cannot follow the changing air path and impact a collection surface. Particles with less inertia remain suspended and continue downstream, allowing the instrument to separate the aerosol according to aerodynamic diameter.
The staged design creates successive aerodynamic separation points rather than one broad collection step. Larger aerodynamic particles are removed earlier, while smaller particles pass to later stages as the nozzle dimensions decrease. Comparing material collected across the stages reveals the particle-size distribution and provides a basis for estimating which respiratory regions particles may reach.
The distribution of material across collection surfaces indicates how the aerosol is partitioned among aerodynamic size ranges. A concentration concentrated on earlier stages suggests a greater contribution from particles with higher inertia, whereas later-stage collection indicates smaller airborne particles. This stage-by-stage pattern supports interpretation of potential respiratory deposition and exposure.
Researchers direct the aerosol through the impactor, allowing the airflow to accelerate through each successive stage. Particles are retained on the appropriate collection surfaces when their inertia prevents them from following the airflow. Material associated with the stages is then used to determine the particle-size distribution and relate it to respiratory deposition or exposure.
Infectious bioaerosol studies use the measured aerodynamic size distribution to characterize airborne particles relevant to transmission and exposure. The results help researchers examine how infectious material may be distributed through an aerosol and support assessment of dose-related conditions. They can also inform evaluation of aerosol-generating procedures in immunology and infection research.
Measurements from a Cascade Impactor show how an inhaled therapeutic or respiratory aerosol is distributed by aerodynamic diameter. That information helps researchers predict where particles may deposit in the respiratory tract and assess whether an intervention produces a relevant airborne profile. These data support improvement of inhalation-based interventions and interpretation of their potential airway delivery.