Chicken Embryonated Eggs
Chicken Embryonated Eggs - Related Videos
06:56
Influenza Virus Propagation in Embryonated Chicken Eggs
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Cited by 105 •
2015
Fertile chicken eggs are widely used to produce large amounts of human influenza A virus as they provide a convenient and cost-effective system to prove high yields of virus.
05:02
Purification of Virus from the Allantoic Fluid of Embryonated Chicken Eggs
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2026
Source: Yates, J. G. E., et al. Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid. J. Vis. Exp. (2022)The video demonstrates the purification and concentration of virus particles from clarified allantoic fluid collected from virus-infected embryonated chicken eggs. It showcases a series of filtration and separation steps, including depth filtration, tangential flow filtration, and density gradient ultracentrifugation to isolate intact viruses. The virus-rich...
15:01
Windowing Chicken Eggs for Developmental Studies
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Cited by 37 •
2007
The ease of accessibility of the embryo has allowed for experiments to map cell fates using several approaches, including chick quail chimeras and focal dye labeling. In this article we demonstrate one egg preparation method that has been optimized for long survival times.
05:08
Egg Windowing: A Method of Opening Fertilized Chicken Egg Shell to Expose Chorioallantoic Membrane Vasculature
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2023
In this video, we describe a technique to open the eggshell of a fertilized chicken egg to expose its chorioallantoic membrane or CAM carrying a network of branching blood vessels. The exposed CAM can be used for in ovo tumor modeling.
03:12
Propagation and Harvest of Influenza Virus from a Fertilized Chicken Egg
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2026
Source: Brauer, R. et al., Influenza Virus Propagation in Embryonated Chicken Eggs. J. Vis. Exp. (2015)This video demonstrates the synthesis and harvest of the influenza virus in fertilized chicken eggs. The virus is inoculated into the allantoic cavity, where it replicates in epithelial cells and buds into the allantoic fluid, which is then collected and stored for downstream applications.
FAQs
Each site provides a different developmental compartment in which an infectious agent can replicate. Researchers may target the chorioallantoic membrane, allantoic cavity, or amniotic cavity, depending on the biological question and the agent being examined. Comparing tissue or embryo effects across these sites helps connect the location of replication with observable aspects of infection.
Separate anatomical compartments allow replication and host effects to be examined within a living developing system rather than as an undifferentiated response. Tissue changes and effects on the embryo provide measurable outcomes, while controlled conditions and defined developmental stages help researchers relate those outcomes to the timing and setting of infection.
They combine a living host environment with accessible anatomy and defined development. This provides biological context that a cell-only system may not capture, while remaining less complex than more advanced animal models. In immunology and infection research, the eggs can therefore connect controlled pathogen studies with questions about tissue effects and host responses.
Researchers begin with fertilized eggs containing developing embryos, select an appropriate inoculation site, and introduce the infectious agent under controlled conditions. They then examine whether replication occurs and record measurable tissue or embryo effects. This workflow can be adapted for viral isolation, propagation, or pathogenesis studies, depending on the intended outcome.
Route selection depends on which compartment is most relevant to the agent or question. The chorioallantoic membrane supports examination of effects in a defined tissue, whereas the allantoic and amniotic cavities provide different sites for pathogen replication. Using a site-specific approach helps researchers obtain interpretable observations rather than treating the egg as a single uniform environment.
They are used to isolate and propagate viruses, investigate pathogenesis, examine host responses, and produce certain vaccines. These applications make the model valuable when researchers need both pathogen-related outcomes and information about the infected host. Its developmental accessibility also supports comparisons across defined stages, adding context to changes observed during infection.