Skin Graft Rejection
Skin Graft Rejection - Related Videos
09:30
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
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Cited by 44 •
2016
The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
07:05
Mouse Model of Alloimmune-induced Vascular Rejection and Transplant Arteriosclerosis
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Cited by 7 •
2015
We describe a protocol for aortic interposition grafting in mice. The goal of the protocol is to provide a model with which to study pathological processes and therapeutic strategies relevant to alloimmune reactions in arteries and the resultant arterial changes that contribute to organ transplant failure.
06:42
Murine Skin Transplantation
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Cited by 36 •
2008
Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c-->C57BL/6 skin transplant.
FAQs
Recipient T lymphocytes recognize donor human leukocyte antigens and other alloantigens on transplanted skin. Their activation initiates cytokine release and attracts inflammatory cells, creating a local immune response rather than a purely mechanical graft failure. This recognition explains why donor-recipient compatibility and immune monitoring matter in transplantation.
Cytokines released by activated recipient T lymphocytes recruit inflammatory cells to the transplanted tissue. These cells can injure both graft blood vessels and skin, producing progressive tissue damage. Because vascular structures are affected, the immune response can be associated with poor graft adherence and tissue necrosis, threatening overall graft viability.
Infection can further compromise healing in an already injured graft. This makes infection a clinically important complication rather than a separate concern: inflammation, impaired graft adherence, and tissue necrosis may worsen while the wound is being assessed. Prompt recognition and treatment of infection therefore support efforts to preserve graft viability.
Monitoring focuses on redness, swelling, pain, impaired graft adherence, and tissue necrosis. These findings provide observable evidence that the graft may be experiencing immune-mediated injury or complications affecting healing. Regular assessment helps clinicians recognize deterioration early, evaluate graft viability, and decide whether wound care, immunosuppression, or infection treatment needs attention.
Management includes careful wound care, immunosuppression, and timely treatment of infection. Wound care supports the local graft environment, while immunosuppression addresses the recipient’s immune response. Treating infection is also important because it can further compromise healing. Together, these measures aim to protect graft viability during clinical monitoring.
Understanding the immune mechanisms behind graft injury helps clinicians consider graft selection, monitor patients, and respond to changes that threaten viability. This knowledge is especially relevant in burn care and reconstructive medicine, where successful healing is central to restoring damaged skin. It also connects immunology and infection management with practical transplantation decisions.