Leukocyte Migration
Leukocyte Migration - Related Videos
07:42
Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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Cited by 8 •
2010
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
05:12
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
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Cited by 11 •
2015
This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.
08:24
The Transwell Migration Assay
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2023
Cells migration in response to chemical cues is crucial to development, immunity and disease states such as cancer. To quantify cell migration, a simple assay was developed in 1961 by Dr. Stephen Boyden, which is now known as the transwell migration assay or Boyden chamber assay. This set-up consists an insert which separates the wells of a multiwell plate into top and bottom compartments. Cells whose migration is to be studied are seeded into the top compartment and the chemoattractant...
10:10
In Vivo Imaging Uncovers the Migratory Behavior of Leukocytes within the Joints
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2025
In vivo imaging of the joints is a valuable technique for uncovering the migratory behavior of leukocytes and molecular dynamics within the inflamed joints. Here, we present a detailed protocol for in vivo imaging of the joints using a murine model of type II collagen-induced arthritis (CIA).
11:30
Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
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Cited by 28 •
2015
The ability of inflamed endothelium to recruit leukocytes from flow is regulated by mesenchymal stromal cells. We describe two in vitro models incorporating primary human cells that can be used to assess neutrophil recruitment from flow and examine the role that mesenchymal stromal cells play in regulating this process.
FAQs
Selectins and integrins support different stages of vessel interaction. Selectins help establish leukocyte rolling along the endothelium, while activated integrins produce the stronger attachment required for firm adhesion. This staged control prevents immediate arrest in the bloodstream and allows inflammatory signals to regulate when and where a leukocyte begins leaving the vessel.
Chemokines provide directional information by forming concentration differences within affected tissue. Leukocytes respond by moving toward higher chemoattractant concentrations rather than dispersing randomly. This guidance connects the initial vascular recruitment process with localization at microbial invasion or injury sites, where cells can coordinate inflammation, phagocytosis, and pathogen clearance.
Transendothelial migration is the transition from firm attachment at the blood-vessel lining to movement into surrounding tissue. It places leukocytes beyond the circulation, where they can follow local chemical gradients. This step is essential because adhesion alone keeps cells at the vessel wall, whereas tissue entry permits direct participation in the response to infection or injury.
Recruitment directs white blood cells from circulation toward locations where microbes are present. Once they reach those sites, the cells contribute to coordinated inflammation, phagocytosis, and pathogen clearance. The process therefore links blood-vessel signaling with local immune action, helping explain how immune responses become concentrated at sites of microbial invasion rather than distributed uniformly across tissues.
Researchers can examine how inflammatory signals control rolling, firm adhesion, vessel exit, and movement along tissue gradients. Comparing these stages helps identify where recruitment succeeds or becomes excessive or misdirected. Such analysis provides a framework for understanding immune defense and inflammatory disease while connecting cellular behavior with the outcome of infection or injury responses.
Leukocyte recruitment is beneficial when it directs cells to infection or injury, but excessive or misdirected movement can contribute to inflammatory disease. Studying the signals and adhesion events that control this process can therefore support therapies designed to regulate recruitment. The goal is to preserve useful immune defense while limiting inappropriate inflammatory activity.