Fimh Adhesin
Fimh Adhesin - Related Videos
01:20
FimH-Mediated Host Interaction of Enterobacter cloacae in a Bladder Epithelial Cell Model
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2026
Take cultures of human urinary bladder epithelial cells in a multiwell plate.Incubate the control culture with wild-type Enterobacter cloacae, a pathogenic bacterium with pili, hair-like structures on the bacterial surface.The FimH subunit of the pili binds to the host cell receptor, anchoring the wild-type bacteria to the host cell.Incubate the experimental culture with a mutant Enterobacter cloacae lacking FimH.Without FimH, the mutant bacteria fail to adhere to the host...
03:04
An Intragastric Gavage Technique for Controlled Helicobacter Infection in Mice
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2025
This video demonstrates the establishment of Helicobacter pylori infection through the intragastric gavage technique in a mouse model. The bacterial suspension is administered via a catheter inserted through the mouth into the stomach. Inoculated bacteria colonize and establish an infection in the stomach, which can be used to study the host-pathogen interaction.
03:10
Micro-Parallel Plate Flow Chamber Model for Studying Bacterial Adhesion to Human Endothelial Cells
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2026
Source: Claes, J., et al, In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions. J. Vis. Exp. (2015).This video demonstrates a method for studying host-pathogen interactions using a micro-parallel plate flow chamber seeded with human primary endothelial cells. Following endothelial cell activation by an ionophore and perfusion with fluorescently labeled bacteria, the cells release von Willebrand factor (VWF), which binds bacterial adhesins under shear...
02:51
Catheter-Associated Urinary Tract Infection in a Mouse Model
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2026
Source: Conover, M. S., et al., J. Establishment and Characterization of UTI and CAUTI in a Mouse Model. J. Vis. Exp. (2015)This video demonstrates the procedure for assembling and implanting a transurethral catheter in a female mouse, followed by bacterial inoculation to establish catheter-associated urinary tract infection and investigate biofilm-mediated host-pathogen interactions.
02:56
An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment
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2025
This video describes the simple ATP-measuring assay using luciferase glow reagent. This assay is used to quantify Neisseria gonorrhoeae survival after treatment with ceftriaxone.
FAQs
Mechanical force can strengthen the FimH–mannose interaction rather than simply disrupt it. This catch-bond behavior helps attached bacteria resist detachment when fluid movement creates stress across the host surface. The mechanism is important because it links physical conditions at epithelial tissues with bacterial persistence, showing how attachment can become more stable during flow.
Mannose-containing glycans provide the host-cell recognition sites required for FimH-mediated attachment. Differences in the presence or accessibility of these glycans can influence where bacteria bind, helping explain tissue tropism, meaning preference for particular host sites. Studying this recognition step connects molecular binding specificity with the distribution and persistence of infection.
FimH offers a defined example of how a bacterial surface component identifies a host-cell feature and converts that recognition into stable attachment. Its position at the tip of type 1 fimbriae also links a specific molecular interaction to the behavior of the whole bacterium. This makes it valuable for examining early colonization and persistence.
Investigating FimH can clarify how bacterial attachment contributes to colonization, persistence, and tissue tropism. Because the interaction depends on mannose recognition and can respond to mechanical force, studies can relate molecular binding to conditions encountered at host surfaces. These findings provide immunology and infection research with a model for analyzing early host-pathogen events.
Anti-adhesion strategies aim to interfere with the attachment step so bacteria cannot remain established on host tissues. This approach focuses on preventing colonization rather than directly killing bacterial cells. Consequently, FimH provides a target for interventions designed to reduce infection by disrupting host-pathogen contact at an early stage.
FimH can serve as a focal point for vaccine and diagnostic strategies because its adhesion function is closely tied to bacterial colonization. Vaccine research can examine it as a target for preventing attachment, while diagnostic approaches can use FimH-associated features to investigate infection-related bacterial presence or behavior. These applications extend its value beyond basic mechanism studies.