Growth Pouches
Growth Pouches - Related Videos
03:47
Developing an Experimental Aneurysm Model in a Rabbit Using an Elastase-Treated Vessel Pouch
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2025
Source: Wanderer, S., et al. Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit. J. Vis. Exp. (2020)This video demonstrates the creation of an experimental aneurysm model in a rabbit using an elastase-treated vessel pouch. After excising a segment of the right CCA, the pouch is treated with elastase, then sutured to the left CCA to restore blood flow. The resulting stress on the weakened pouch walls induces aneurysm formation.
03:41
An Air Pouch Mouse Model for Lipopolysaccharide-Induced Inflammatory Exudate Collection
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2025
This video describes the procedure for generating an air pouch in the subcutaneous tissue of mice and collecting inflammatory exudates in response to lipopolysaccharide administration in vivo. This video also covers the analysis of the neutrophil infiltration in the air pouch.
07:34
Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects
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Cited by 11 •
2011
An optimized technique for the microsurgical creation of arterial bifurcation aneurysms mimicking bifurcation human cerebral aneurysms is described. A venous pouch is sutured into an artificially created true bifurcation of both common carotid arteries. Facilitated microsurgical techniques and aggressive postoperative anticoagulation and analgesia lead to minimized morbidity rates and high aneurysm patency rates.
10:45
In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
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Cited by 4 •
2021
This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.
06:28
Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
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Cited by 22 •
2018
This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.
FAQs
The transparent pouch surface allows researchers to follow developing roots directly as they grow along the supporting layers. This makes root development visible without relying only on endpoint observations. In infection studies, the same visibility helps investigators examine where microorganisms interact with roots and relate colonization patterns to changes in growth over time.
Seeds are positioned between moist paper or comparable supporting layers, creating a surface through which water and nutrients can reach the developing roots. Microbial inocula can also be introduced to this root environment. Their access to the roots supports controlled examination of how beneficial or pathogenic microorganisms establish interactions with plant tissues.
They connect visible root development with the plant microorganism interaction occurring at the root surface. Researchers can examine root colonization while also considering host defense responses and factors associated with susceptibility or resistance. This provides a practical laboratory setting for linking infection-related interactions with observable changes in developing roots.
A typical workflow begins by placing seeds between moist paper or other supporting layers inside the sterile pouch. Researchers then maintain the seeds under controlled laboratory conditions as roots develop and introduce microbial inocula when examining colonization or infection. During growth, they observe the roots and collect appropriate root or exudate samples.
The system supports collection of root samples and exudates, which are materials released from roots into their surrounding environment. These samples can be examined alongside direct observations of root development and microbial interaction. Together, they provide information about the developing root and the local environment in which colonization or host responses occur.
Growth Pouches provide the same visible root environment for examining interactions with either beneficial or pathogenic microorganisms. Researchers can monitor how roots develop, assess patterns of root colonization, and collect root or exudate samples. These observations help investigate how microbial interactions relate to plant defense, susceptibility, or resistance under controlled laboratory conditions.