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Journal / Neuroscience / In Situ Visualization of Axon Growth and Growth…
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Neuroscience

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JoVE Journal Neuroscience
In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
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In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

Article DOI: 10.3791/63068-v • 10:45 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1
1Laboratory of Axon Growth and Regeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

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Summary
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This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.

Tags

Axon Growth Growth Cone Dynamics Ex Vivo Brain Slice Cultures In Situ Visualization Neurobiology High Resolution Imaging DNA Delivery Organotypic Slices Image Acquisition And Analysis Axon Plasticity Traumatic Conditions Pathological Conditions Electroporation Brain Dissection Slicing Steps Pregnant Female Mouse Uterine Horns Wet Gauze HBSS Supplementation
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