Keratin 14 Staining
Keratin 14 Staining - Related Videos
14:43
Synthesis of Keratin-based Nanofiber for Biomedical Engineering
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Cited by 12 •
2016
Electrospun nanofibers have a high surface area to weight ratio, excellent mechanical integrity, and support cell growth and proliferation. These nanofibers have a wide range of biomedical applications. Here we fabricate keratin/ PCL nanofibers, using the electrospinning technique, and characterize the fibers for possible applications in tissue engineering.
08:29
Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics
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Cited by 23 •
2017
The goal of the protocol is to prepare keratin hydrolysate from chicken feathers by alkaline-enzymatic hydrolysis and to test whether adding keratin hydrolysate into a cosmetics ointment base improves skin barrier function (heightening hydration and diminishing transepidermal water loss). Tests are conducted on men and woman volunteers.
07:59
Airfoil Behavior: Pressure Distribution over a Clark Y-14 Wing
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2023
Source: David Guo, College of Engineering, Technology, and Aeronautics (CETA), Southern New Hampshire University (SNHU), Manchester, New Hampshire An airfoil is a 2-dimensional wing section that represents critical wing performance characteristics. The pressure distribution and lift coefficient are important parameters that characterize the behavior of airfoils. The pressure distribution is directly related to the lift generated by airfoils. A Clark Y-14 airfoil, which is used in this...
11:16
Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
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Cited by 59 •
2009
This video shows a procedure for isolating intact protoplasts from tissues of 14-day-old seedlings of Arabidopsis. Given that the isolated protoplasts remain intact for at least 96h and are isolated from seedlings instead of one-month-old mature plants, this procedure expedites assays requiring intact protoplasts.
11:33
Microscopy and Staining: Gram, Capsule, and Endospore Staining
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2023
Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...
FAQs
The primary antibody provides molecular recognition by binding keratin 14, while the labeled secondary antibody converts that binding event into a visible fluorescent or chromogenic signal. This two-antibody arrangement allows investigators to locate K14 within a specimen and examine how its distribution relates to epithelial organization, integrity, or altered tissue structure.
Because K14 is concentrated in basal epithelial cells, its staining pattern provides a structural reference for evaluating the epithelial compartment. Changes in the location or distribution of signal can help reveal basal-cell responses, epithelial injury, and repair-associated remodeling, rather than being interpreted as an isolated marker unrelated to tissue architecture.
Both readouts reveal where the secondary antibody has detected K14, but they present the result differently. Fluorescent labeling displays signal through fluorescence, whereas chromogenic labeling produces a visible color reaction. The selected format therefore affects how investigators visualize and document K14 distribution in fixed tissue or cultured cells.
A typical workflow uses fixed tissue or cultured cells, applies a primary antibody that binds K14, and then adds a labeled secondary antibody to generate the detectable signal. Investigators subsequently examine the fluorescent or chromogenic pattern to map K14 distribution and relate it to epithelial structure, integrity, or disease-associated remodeling.
K14 staining supplies an epithelial map against which immune-cell positions can be interpreted. In immunology studies, researchers can compare the basal epithelial pattern with the distribution of immune cells, helping distinguish changes associated with epithelial injury or repair from the broader spatial organization of the affected tissue.
In infection research, the assay can help assess epithelial damage and tissue remodeling associated with disease or pathogen-associated injury. Its value is contextual: the K14 pattern identifies changes in the epithelial compartment, allowing investigators to relate structural disruption and repair responses to immune activity within the same tissue setting.