3d Lung Tissue Model
3d Lung Tissue Model - Related Videos
10:10
A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
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Cited by 40 •
2015
Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.
05:47
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
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Cited by 46 •
2019
Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.
13:34
A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
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Cited by 29 •
2016
We present a three-dimensional (3D) lung cancer model based on a biological collagen scaffold to study sensitivity towards non-small-cell-lung-cancer-(NSCLC)-targeted therapies. We demonstrate different read-out techniques to determine the proliferation index, apoptosis and epithelial-mesenchymal transition (EMT) status. Collected data are integrated into an in silico model for prediction of drug sensitivity.
11:12
Tissue Engineering of a Human 3D in vitro Tumor Test System
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Cited by 34 •
2013
Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
04:35
Ex vivo Pig Lung Model of Biofilm: A Technique to Develop Lung Model to Study Bacterial Biofilms on Pig Bronchiolar Tissue
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2025
This video describes the protocol for developing an ex vivo pig lung model of bacterial biofilm on infected bronchiolar tissue. Biofilms are bacterial aggregates embedded in a matrix adherent to a surface. This model is used to study the antibiotic susceptibility of bacteria at different stages of biofilm formation.
FAQs
Epithelial cells provide the lung-facing cellular surface, while the extracellular matrix supplies a surrounding structural context that supports cell interactions and barrier behavior. Their combination allows investigators to examine how lung tissue responds to environmental stimuli in a setting that better represents three-dimensional organization than a flat culture. This is particularly useful when studying tissue responses during infection or injury.
Adding immune cells can extend the model beyond epithelial responses by supporting investigation of host defense, inflammation, and tissue injury. Some systems also include vascular cells, enabling researchers to examine additional cell interactions within the tissue environment. Because these components are optional rather than universal, their inclusion should match the biological question, such as studying infection-associated inflammation or immune responses.
Three-dimensional organization provides a more physiologically relevant setting for examining interactions among lung cells, extracellular matrix, and, when included, immune or vascular cells. This can reveal barrier responses, pathogen-related effects, and inflammatory changes that are difficult to interpret in a simpler flat culture. The added tissue context makes the model valuable for investigating respiratory disease mechanisms.
These systems can support separate examination of pathogen entry, replication, inflammation, tissue injury, and host defense within a controlled model. Considering these responses together helps connect early interactions between a pathogen and lung tissue with later damage or protective activity. The approach therefore supports more integrated analysis of respiratory infection mechanisms than measuring a single cellular outcome alone.
A basic system combines lung epithelial cells with extracellular matrix, creating the cellular and structural foundation for tissue-level analysis. Depending on the research goal, investigators may also incorporate immune or vascular cells to study host defense, inflammation, or broader cell interactions. The selected components determine which aspects of barrier function, infection, or tissue response the model can address.
Investigators can expose the modeled lung tissue to environmental stimuli associated with infection and then examine pathogen entry, replication, inflammation, tissue injury, and host defense. Because the system maintains relevant cellular interactions in a controlled setting, it helps connect pathogen behavior with changes in lung tissue. This supports mechanistic studies of respiratory disease and host-pathogen interactions.
The models provide a controlled tissue setting for evaluating how vaccines, antimicrobial treatments, or immunotherapies influence infection-related responses. Researchers can assess effects on pathogen-associated processes, inflammation, tissue injury, or host defense rather than relying only on isolated cellular measurements. These applications may help compare treatment responses while improving understanding of respiratory disease mechanisms.