Neuropathological Assessment
Neuropathological Assessment - Related Videos
07:30
A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments
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Cited by 11 •
2017
This report outlines a simple approach to successfully induce experimental autoimmune neuritis (EAN) using the myelin protein zero (P0)180-199 peptide in combination with Freund's complete adjuvant and pertussis toxin. We present a sophisticated paradigm capable of accurately assessing the extent of functional deficits and neuropathology that occur in this EAN.
10:02
Assessment of Spontaneous Alternation, Novel Object Recognition and Limb Clasping in Transgenic Mouse Models of Amyloid-β and Tau Neuropathology
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Cited by 199 •
2017
Described here is a staged, behavioral screening approach that can be used to screen for compounds that exhibit in vivo efficacy on cognitive and functional motor behaviors in transgenic mouse models of β-amyloidosis and tauopathy. These methods are optimized to screen compounds for activity in short-term and working memory tasks.
08:39
Assessing Cortical Cerebral Microinfarcts on High Resolution MR Images
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Cited by 24 •
2015
A high resolution ex vivo 7T MR imaging protocol is presented, to perform MR-guided histopathological validation of microvascular pathology in post-mortem human brain tissue. Furthermore, guidelines are provided for the assessment of cortical microinfarcts on in vivo 7T as well as 3T MR images.
08:29
Symmetric Bihemispheric Postmortem Brain Cutting to Study Healthy and Pathological Brain Conditions in Humans
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Cited by 7 •
2016
Organized brain cutting procedures are necessary to correlate specific neuropsychiatric phenomena with definitive neuropathologic diagnoses. Brain cuttings are performed differently based on various clinico-academic contingencies. This protocol describes a symmetric bihemispheric brain cutting procedure to investigate hemispheric differences in human brain pathologies and to maximize current and future biomolecular/neuroimaging techniques.
08:36
Neurotoxicity Assessment in Adult Danio rerio using a Battery of Behavioral Tests in a Single Tank
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Cited by 3 •
2023
Here, we present a comprehensive behavioral test battery, including the novel tank, Shoaling, and social preference tests, to effectively determine the potential neurotoxic effects of chemicals (e.g., methamphetamine and glyphosate) on adult zebrafish using a single tank. This method is relevant to neurotoxicity and environmental research.
FAQs
Gross examination provides an initial view of tissue distribution and visible abnormalities, while microscopy resolves cellular architecture and lesion patterns. Histological stains add structural detail, immunohistochemistry identifies selected cellular or pathogen-associated targets, and molecular testing supplies additional evidence when morphology alone is insufficient. Combining these levels helps localize disease and characterize its biological effects more completely.
Immunohistochemistry can help identify particular targets within affected tissue, allowing investigators to relate pathogen-associated signals to immune-cell infiltration, edema, vascular injury, or tissue degeneration. This spatial information is important because it shows where relevant changes occur rather than only indicating that an abnormality is present. The results can therefore connect tissue injury with infectious or immune processes.
The assessment can document several distinct consequences, including the presence of infectious agents, accumulation of immune cells, edema, vascular injury, and degeneration of nervous-system tissue. Considering these findings together helps distinguish direct evidence of infection from associated host responses and tissue damage. Their distribution across the brain, spinal cord, or peripheral nerves also contributes to lesion localization.
A typical sequence begins with gross examination, followed by microscopic evaluation of tissue structure. Histological staining can then clarify cellular and architectural changes, while immunohistochemistry adds target-specific localization. Molecular testing is used when needed to provide further characterization. This staged approach allows investigators to obtain increasingly specific information without relying on a single type of evidence.
It provides tissue-level evidence linking immune-cell infiltration and other inflammatory changes with the location and severity of lesions. Researchers can examine whether edema, vascular injury, or degeneration accompanies those responses and how the pattern relates to infectious findings. This makes the assessment useful for investigating disease mechanisms in immunology and infection rather than evaluating immune activity in isolation.
In experimental models, tissue findings can show whether a disease process produces the expected nervous-system lesions and associated inflammatory or infectious changes. The same framework supports assessment of antimicrobial or immune-modulating treatments by comparing pathological outcomes across conditions. Changes in pathogen-associated findings, immune infiltration, edema, vascular injury, or degeneration can help indicate how treatment affects disease-related tissue damage.