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A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments
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Neuroscience
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JoVE Journal Neuroscience
A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments

A Simple Approach to Induce Experimental Autoimmune Neuritis in C57BL/6 Mice for Functional and Neuropathological Assessments

DOI:
10.3791/56455-v

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07:30 min

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November 09, 2017

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, , , , ,
1Department of Anatomy and Neuroscience, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences,The University of Melbourne

Chapters

  • 00:05Title
  • 01:02Experimental Autoimmune Neuritis (EAN) Motor Function Testing and Clinical Scoring
  • 04:11Results: Representative Analysis of P0180-199 Peptide-induced EAN Pathology
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

This report outlines a simple approach to successfully induce experimental autoimmune neuritis (EAN) using the myelin protein zero (P0)180-199 peptide in combination with Freund's complete adjuvant and pertussis toxin. We present a sophisticated paradigm capable of accurately assessing the extent of functional deficits and neuropathology that occur in this EAN.

Tags

Keywords: Experimental Autoimmune NeuritisEANMouse ModelPeripheral NeuropathyMotor Function AssessmentPertussis ToxinMycobacterium TuberculosisComplete Freund’s AdjuvantGait Function Imaging SystemClinical Scoring

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