Vortex Lysis
Vortex Lysis - Related Videos
03:12
A Procedure for Bacterial Harvesting and Mechanical Lysis
0 Views •
2025
Source: Mercier, N., et al. MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria. J. Vis. Exp. (2021)This video demonstrates a step-by-step procedure for harvesting bacterial cells and mechanically lysing them to release intracellular contents.
06:35
Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water
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2025
This study presents the methodology for the generation of six different types of plasma discharges within a Hyperbolic Vortex Plasma Reactor for the degradation of micropollutants in water, including pharmaceuticals and per- and polyfluoroalkyl substances (PFAS).
01:32
Tissue Homogenization and Cell Lysis
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2023
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods. Mechanical methods of tissue homogenization These methods rely on applying external physical force to disrupt...
04:11
Isolation of Endospores from an Environmental Sample by Targeted Lysis of Vegetative Cells
0 Views •
2025
Source: Wunderlin, T., et al. Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells. J. Vis. Exp. (2016)This video demonstrates a method to isolate endospores from an environmental sample containing both vegetative cells and various bacteria. The procedure involves filtration, mechanical disruption, and selective chemical treatment to weaken and lyse vegetative cells while preserving endospores. The final step isolates the resilient endospores through...
09:53
Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
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Cited by 4 •
2009
An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.
FAQs
Rapid agitation subjects the suspended sample to mechanical stress, while moving beads add repeated collisions against cells or microbial material. Together, these forces can rupture cell envelopes and membranes, releasing intracellular contents into the surrounding buffer. The resulting lysate makes otherwise enclosed proteins, nucleic acids, antigens, and other microbial components available for subsequent analytical or immunological measurements.
Beads provide an additional mechanical source of disruption during vigorous mixing. As the sample moves around them, bead-sample collisions supplement the shear forces generated by agitation and help rupture cellular or microbial structures. This combination can improve exposure of intracellular material, making bead-assisted processing useful when downstream assays require access to proteins, nucleic acids, antigens, or microbial components.
The sample is processed while suspended in an appropriate lysis buffer, which provides the liquid context for agitation and collection of released intracellular material. Because the buffer becomes part of the lysate, its suitability matters for the intended downstream workflow, including protein analysis, nucleic-acid detection, immunoassays, or pathogen characterization.
A typical workflow begins by placing the biological sample in an appropriate lysis buffer. Beads may then be added before the tube is subjected to vigorous vortexing, allowing shear forces and bead-sample collisions to disrupt the material. The resulting lysate can proceed to protein analysis, nucleic-acid detection, immunoassays, or pathogen-characterization procedures.
The released material supports several types of analysis rather than a single readout. Protein analysis can examine intracellular proteins, while nucleic-acid detection accesses genetic material released during disruption. Immunoassays can evaluate exposed intracellular antigens, and pathogen-characterization workflows can examine microbial components. The useful outcome therefore depends on the downstream test applied to the lysate.
In these fields, important targets may remain inside host cells or microorganisms until the sample is disrupted. Vortex lysis helps expose intracellular antigens and microbial components for immunological or molecular testing. Its rapid processing and simple equipment also suit routine sample preparation, particularly when workflows require consistent disruption before protein analysis, nucleic-acid detection, or pathogen characterization.