Bat Quantification
Bat Quantification - Related Videos
00:07
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Cited by 10 •
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Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
15:31
Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture
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08:44
Invertebrate Lifespan Quantification
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Many animals naturally stop growing upon reaching adulthood, after which they undergo aging or "senescence" until dying. The amount of time between an organism\'s birth and death is called its lifespan, which can be influenced by various biological and environmental factors. By exposing organisms to different growth conditions, scientists can better understand the factors affecting lifespan. Flies and worms are ideal organisms to perform such experiments, given their short generation time and...
08:38
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
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Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.
FAQs
Cold exposure increases sympathetic stimulation, which activates brown adipose tissue and raises its glucose uptake. This metabolic response creates a stronger signal for 18F-fluorodeoxyglucose positron emission tomography, making BAT easier to identify than under conditions without stimulation. The resulting images can therefore support estimates of both tissue activity and the amount of BAT present.
Glucose uptake serves as an image-based indicator of BAT metabolic activity after sympathetic stimulation. Increased uptake produces a detectable signal that helps distinguish active tissue and supports quantitative assessment. Because the measurement reflects activity as well as tissue presence, researchers can examine functional changes rather than relying only on anatomical tissue volume.
The two measurements describe different features of the same tissue. Image signals can be used to estimate BAT volume, representing how much tissue is present, while glucose uptake provides information about metabolic activity. Considering both dimensions helps researchers determine whether a condition or intervention is associated with altered tissue quantity, altered function, or both.
Researchers can use these measurements to study how medications, environmental conditions, and metabolic disorders influence BAT function. The approach is relevant to investigations of obesity, diabetes, metabolism, and thermoregulation. Comparing BAT activity or volume across such conditions can reveal whether changes in heat production and energy expenditure accompany broader metabolic differences.
A supported workflow begins with cold exposure to stimulate BAT and increase glucose uptake. Researchers then acquire combined 18F-fluorodeoxyglucose positron emission tomography and computed tomography images. The images help identify the tissue, while their signals are analyzed to estimate BAT metabolic activity and volume. These measurements provide the quantitative outcomes used for subsequent comparisons.
The method is useful when a study needs to evaluate BAT involvement in energy expenditure, heat production, or metabolic regulation. In medicine, applications include research on obesity and diabetes, investigations of thermoregulation, and assessment of how medications or environmental conditions affect BAT. It can also support comparisons involving metabolic disorders and their effects on tissue function.