Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Laurel R. Yohe; Paolo Devanna; Kalina  T.J. Davies; Joshua H.T. Potter; Stephen  J. Rossiter; Emma  C. Teeling; Sonja C. Vernes; Liliana  M. D&#225;valos

doi:10.3791/59505

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

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Biology

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JoVE Journal Biology

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

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15:31 min

October 23, 2019

DOI: 10.3791/59505-v

Laurel R. Yohe*^1,2, Paolo Devanna*³, Kalina T.J. Davies⁴, Joshua H.T. Potter⁴, Stephen J. Rossiter⁴, Emma C. Teeling⁵, Sonja C. Vernes*^3,6, Liliana M. Dávalos*^2,7

¹Department of Geology & Geophysics,Yale University, ²Department of Ecology & Evolution,Stony Brook University, ³Neurogenetics of Vocal Communication,Max Planck Institute for Psycholinguistics, ⁴School of Biological and Chemical Sciences,Queen Mary University of London, ⁵School of Biology & Environmental Science,University College Dublin, ⁶Donders Institute for Brain, Cognition and Behavior, ⁷Consortium for Inter-Disciplinary Environmental Research,Stony Brook University

Overview

The study presents a protocol for optimal tissue preparation essential for genomic, transcriptomic, and proteomic analyses of wild-caught bats. It emphasizes humane bat capture and meticulous dissection to maximize the quality of collected molecular data while minimizing impact on bat populations.

Key Study Components

Research Area

Genomic analyses
Transcriptomic studies
Proteomic profiling

Background

Importance of tissue preservation in molecular biology
Non-lethal sampling methods for bats
Advancements in techniques for tissue collection and storage

Methods Used

Step-by-step humane capture of bats
Dissection and preservation techniques for various tissues
Use of RNA stabilizing solutions for molecular preservation

Main Results

Detailed protocols for dissection that enhance the quality of collected data
Successful preservation methods for multiple tissue types ensuring their viability for future studies
Generated sufficient material for both genomic and functional studies without lethal sampling

Conclusions

Underscores the necessity of non-lethal methods for sampling wild animals
Provides a framework for effective molecular studies in bat populations, crucial for ecological research

Frequently Asked Questions

What is the main goal of this tissue preparation protocol?

The protocol aims to optimize tissue collection for downstream genomic, transcriptomic, and proteomic analyses.

Why is humane capture important in this research?

Humane capture minimizes the impact on bat populations while allowing for valuable scientific data collection.

What types of tissues can be collected using this protocol?

Various tissues including brain, muscle, lung, heart, liver, and reproductive organs can be collected.

How are tissues preserved after collection?

Tissues are preserved in RNA stabilizing solutions or flash-frozen in liquid nitrogen for future analysis.

What is the advantage of using non-lethal sampling methods?

Non-lethal methods allow for ongoing studies of the same populations without depleting their numbers.

Can this protocol be applied to other species?

While this protocol is tailored for bats, similar principles can be adapted for other wildlife studies.

What technologies are essential for this protocol?

Essential technologies include RNA stabilization solutions and methods for preserving high molecular weight DNA.

This is a protocol for the optimal tissue preparation for genomic, transcriptomic, and proteomic analyses of bats caught in the wild. It includes protocols for bat capture and dissection, tissue preservation, and cell culturing of bat tissue.

In this video, we provide a step by step guide to collecting bats in a humane way, intended to minimize impact on populations and maximize scientific value. The advantages of our approach are that we maximize the amount of potential molecular and morphological data for each bat, preserve tissues to retain their future value, and minimize harvesting wild individuals. Dissection protocols are difficult to verbally demonstrate.

Many of the tissue preparation techniques we present here are best communicated through visualizing how different organs are identified, and then properly dissected and stored. Prepare all vials before starting any dissections to avoid delays. For RNA, set aside the number of vials needed for each specimen.

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