Casa System
Casa System - Related Videos
11:05
High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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Cited by 30 •
2015
Fourier Transform Infrared (FT-IR) spectroscopic imaging is a fast and label-free approach to obtain biochemical data sets of cells and tissues. Here, we demonstrate how to obtain high-definition FT-IR images of tissue sections towards improving disease diagnosis.
08:58
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
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Cited by 2 •
2015
Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.
09:09
Large-Scale Production of Extracellular Vesicles from High-Density 3D Cell Cultures in Hollow Fiber Perfusion Bioreactors
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2026
Instructions are provided for setting up and using a 3D perfusion hollow fiber bioreactor for extracellular vesicle production from MSC and other cell types. This protocol describes a method for producing extracellular vesicles at high concentrations on a continuous basis while controlling cell density in hollow fiber bioreactors.
11:08
Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
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Cited by 15 •
2012
Lentiviral expression vectors are the most effective vehicles for stably expressing different effector molecules or reporter constructs in dividing and non-dividing mammalian cells and whole organisms. Here we provide a protocol on how to package lentivector expression constructs in pseudoviral particles and to transduce target cells using the pseudoviral particles.
07:03
In Situ Surface Temperature Measurement in a Conveyor Belt Furnace via Inline Infrared Thermography
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Cited by 3 •
2020
This protocol describes how to install an infrared camera into a conveyor belt furnace, conduct a customer correction of a factory calibrated IR camera, and evaluate the spatial surface temperature distribution of an object of interest. The example objects are industrial silicon solar cells.
FAQs
Digital image processing converts recorded microscope frames into tracks for individual sperm cells. The software identifies cells across successive images, then uses those tracks to quantify movement-related measures such as motility, velocity, and motion patterns. Because the same recorded field can be processed systematically, the method reduces reliance on visual estimates and supports more reproducible semen assessment.
Calibration and instrument settings directly affect whether sperm are detected and how their movement is calculated. The sample chamber must provide a consistent viewing environment, while the camera and software settings need to be standardized for comparisons. Without controlled handling and settings, measured concentration, motility, or velocity may vary because of analysis conditions rather than differences in the semen sample.
Some CASA systems evaluate sperm morphology in addition to concentration and movement. Morphology refers to the observed form or structural appearance of individual sperm cells. Including this measurement broadens the assessment of semen quality, allowing a laboratory to consider cell appearance alongside quantitative movement patterns when investigating male fertility or characterizing samples for clinical use.
CASA complements manual examination by adding digital, quantitative measurements to laboratory assessment. Manual review can provide direct visual evaluation, whereas computer-assisted analysis records and processes sperm movement using defined imaging and software conditions. Using both approaches can help clinicians compare objective measures with conventional examination when investigating infertility, monitoring treatment-related changes, or evaluating reproductive samples.
A laboratory places the semen sample in a calibrated chamber, records sperm movement with a microscope-mounted camera, and processes the images with tracking software. The system then reports measurements such as concentration, motility, velocity, and related motion patterns; morphology may also be assessed when supported. Consistent sample handling and instrument settings are necessary throughout the workflow.
Clinicians may use CASA measurements when investigating male infertility and need quantitative information about semen quality. The results can describe how many sperm are present, how actively they move, and which movement patterns occur. These data support assessment alongside other clinical information rather than replacing the broader medical evaluation of fertility.
CASA can help characterize semen samples used in assisted reproductive procedures by providing standardized measurements of concentration and sperm movement, with morphology available on some systems. Repeated analyses can also document changes during treatment. The value of comparisons depends on consistent sample handling, chamber calibration, and instrument settings so that observed differences more likely reflect the samples.