Diaphragm Ultrasound
Diaphragm Ultrasound - Related Videos
07:12
Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
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Cited by 7 •
2014
Diagnostic ultrasound imaging has proven to be effective in diagnosing various respiratory diseases in human and animal subjects. We demonstrate a comprehensive ultrasound protocol utilized by Dr. Zuo's lab to analyze diaphragm kinetics specifically in mouse models. This is also a non-invasive research technique which can provide quantitative information on mouse respiratory muscle function.
05:51
Measuring Diaphragm Thickness and Function Using Point-of-Care Ultrasound
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Cited by 9 •
2023
Diaphragm thickness and function can be assessed in healthy individuals and critically ill patients using point-of-care ultrasound. This technique offers an accurate, reproducible, feasible, and well-tolerated method for evaluating diaphragm structure and function.
09:43
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
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Cited by 16 •
2015
Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.
09:00
Murine Echocardiography and Ultrasound Imaging
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Cited by 61 •
2010
This video demonstrates use of a rail-mounted high-frequency ultrasound probe to perform echocardiography on an anesthetized mouse. The methods describe both conventional two-dimensional and M-mode measurements of cardiac function in addition to newer, more powerful tools such as color Doppler, strain analysis, as well as general and targeted contrast imaging.
02:48
Fluorescence Imaging of Calcium Dynamics at Neuromuscular Junctions in the Diaphragm of a Transgenic Mouse
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2025
Source: Heredia, D. J., et. al. Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm. J. Vis. Exp. (2018) This video demonstrates fluorescence imaging of calcium dynamics at neuromuscular junctions (NMJs) in a transgenic mouse expressing genetically encoded calcium indicators. A NJM is identified by fluorescently tagged acetylcholine receptors in the diaphragm with the phrenic nerve. An image splitter is used to visualize multiple signals...
FAQs
Diaphragmatic excursion describes the diaphragm’s movement during breathing. Observing this movement in real time gives clinicians a functional measure that complements structural measurements, helping them recognize reduced respiratory muscle performance or possible diaphragmatic weakness. Because the assessment can be repeated at the bedside, changes in movement can be followed during critical illness or recovery.
Thickness and thickening fraction add structural and functional information to the movement seen as excursion. Examining these measurements together gives a broader view of diaphragmatic function than relying on movement alone. This combined assessment can help identify impaired respiratory muscle performance and follow whether function changes during illness, ventilator dependence, or treatment.
B-mode and M-mode are the two imaging modes identified for diaphragm ultrasound measurements. Using these modes, clinicians can assess diaphragmatic excursion, muscle thickness, and thickening fraction as part of a functional examination. The important outcome is a real-time evaluation of respiratory muscle performance, rather than a purely structural image.
Clinicians place the probe in a subcostal or intercostal region and observe the diaphragm during breathing. This positioning supports real-time visualization of movement while enabling measurements of excursion, muscle thickness, and thickening fraction. The bedside approach allows functional assessment without radiation and can be repeated when ongoing monitoring is needed.
It is particularly useful when clinicians need repeated information about respiratory muscle performance in critical illness, neuromuscular disorders, or ventilator dependence. The examination can identify diaphragmatic weakness and track performance over time. Its bedside, radiation-free nature makes it suitable for serial assessment during changing clinical conditions.
Measurements of excursion, thickness, and thickening fraction provide information about respiratory muscle performance in patients who depend on mechanical ventilation. Clinicians can use this functional information when assessing readiness for weaning and can repeat the examination to monitor changes or treatment response. It therefore complements ongoing bedside evaluation without exposing the patient to radiation.