Ex Vivo Tissue Model
Ex Vivo Tissue Model - Related Videos
06:30
An ex vivo Mouse Model for High-Fat Diet-Like Adipose Tissue Inflammation
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2026
Using an ex vivo explant model, small pieces of the rostral portion of visceral white adipose tissue from lean mice are cultured for 7–10 days, enabling the induction and study of adipose tissue inflammation as it occurs during high-fat diet feeding.
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
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Cited by 66 •
2013
Here, we present a systematic approach for developing physiologically relevant, sensitive and specific in vivo assays for interpreting variation in human pathology. Transient genetic manipulation via microinjection of WT and mutant human mRNA and morpholino (MO) antisense oligonucleotides harness the tractability of the developing zebrafish embryo to rapidly assay pathogenic mutations, especially, but not exclusively, in the context of human developmental disorders.
07:29
Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model
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2024
The proposed protocol entails a global approach to assess bone formation in the context of bone regeneration using multimodal analyses. It aims to provide qualitative and quantitative information on new bone formation, enhancing the rigor and validity of basic and pre-clinical investigations.
10:00
2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
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2025
Sprouting angiogenesis, fundamental for development and disease, involves complex molecular and mechanical processes. We present a versatile 2.5D ex vivo model that analyzes cellular sprouting from porcine carotid arteries, revealing stiffness-dependent angiogenesis and distinct leader-follower cell mechanics. This model aids in advancing tissue engineering strategies and cancer therapy approaches.
05:00
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Cited by 4 •
2010
This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.
FAQs
Preserved architecture maintains the spatial relationships among cells and the surrounding tissue structure. Those relationships help researchers observe how cellular interactions contribute to normal function or change during disease, injury, or repair. Compared with systems that isolate cells from their tissue context, this organization can provide biologically relevant evidence about responses that depend on communication within the tissue.
Nutrients, oxygen, temperature, and signaling factors are central conditions because they support continued tissue activity outside the organism. If these conditions are appropriate, key cellular interactions and physiological responses can continue during the experiment. Researchers can therefore examine tissue behavior under controlled conditions while relating observed changes to disease processes, treatment effects, or repair responses.
Cellular communication helps preserve coordinated tissue behavior rather than limiting analysis to the actions of individual cells. Maintaining these interactions allows researchers to assess physiological responses in a setting that retains aspects of native biology. This is particularly relevant when investigating disease mechanisms or tissue injury, where changes may involve communication among multiple cellular components.
The model provides controlled biological evidence from preserved tissue while reducing reliance on whole-animal studies. Researchers can focus on tissue structure, cellular communication, and physiological responses without examining an entire organism. This focused approach supports investigation of disease, therapeutic effects, and injury or repair, while retaining aspects of native tissue biology that simpler laboratory systems may not capture.
Researchers remove the tissue from the organism and place it in culture media containing appropriate nutrients and signaling factors. They then maintain suitable oxygen and temperature conditions so the tissue can continue supporting key cellular interactions and physiological responses. Under these controlled conditions, investigators can examine structural or functional changes associated with disease, treatments, devices, injury, or repair.
Medical researchers can use these models to investigate disease mechanisms, evaluate drug or device effects, and study tissue injury and repair. Because the tissue retains aspects of its native architecture and cell communication, results may offer biologically relevant evidence for treatment development. The approach can also support personalized treatments by providing tissue-level information under controlled laboratory conditions.