Laser-assisted Phosphorimetry
Laser-assisted Phosphorimetry - Related Videos
08:59
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
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Cited by 25 •
2016
This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.
07:30
Transcanalicular Diode Laser-assisted Dacryocystorhinostomy for the Treatment of Primary Acquired Nasolacrimal Duct Obstruction
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Cited by 11 •
2017
The goal of this protocol is to present transcanalicular laser-assisted dacryocystorhinostomy as a minimally invasive approach in the treatment of primary acquired nasolacrimal duct obstruction.
09:31
Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types
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Cited by 19 •
2016
Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.
01:08
Matrix-Assisted Laser Desorption Ionization (MALDI)
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2025
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
03:31
Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery
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2025
In this video, we demonstrate the laser-assisted permeation of the protective layer of zona pellucida in mouse fertilized eggs for facilitating lentiviral gene delivery. Lentivirus enables the generation of transgenic animals with a gene of interest stably integrated into their genome.
FAQs
The oxygen-sensitive phosphor provides the connection between local oxygen conditions and the measured signal. After laser excitation, oxygen-dependent quenching changes the phosphor’s emission lifetime, meaning the duration of delayed light emission carries quantitative information. Researchers can use that lifetime change to calculate local oxygen concentration or tissue oxygen tension in biological samples.
The timing of the delayed emission supplies a measurable variable that responds to the surrounding oxygen environment. Rather than relying only on whether light is produced, the method records how the emission lifetime changes after excitation. This supports quantitative assessment of oxygen conditions in tissues, blood, and cell systems, where oxygen availability may vary locally.
Measurements can describe oxygen concentration in a local chemical or biological environment or tissue oxygen tension in medical samples. These outputs connect the phosphorescence response with the oxygenation state of tissues, blood, or cells. Consequently, the technique can support studies of localized oxygen changes rather than only broad assessments of whole-system oxygenation.
A typical measurement uses a laser pulse to activate an oxygen-sensitive phosphor in the sample. The resulting phosphorescence is recorded after excitation, with attention to its emission lifetime. That lifetime is then related to oxygen-dependent quenching to calculate local oxygen concentration or tissue oxygen tension, producing a quantitative oxygenation measurement.
Researchers may select laser-assisted phosphorimetry when they need noninvasive or minimally invasive information about oxygenation in tissues, blood, or cell systems. Its use is especially relevant when local oxygen conditions matter, such as in investigations of hypoxia, circulation, or metabolism. The approach can therefore examine oxygenation without relying solely on broad systemic measurements.
In medicine, the measurements help characterize hypoxia and evaluate circulation and metabolism. They can also support studies of disease mechanisms by showing how oxygenation relates to biological changes. In treatment research, oxygen measurements may help assess treatment response and contribute to the evaluation or development of therapeutic technologies affected by tissue oxygen conditions.