Intravitreal Plasminogen
Intravitreal Plasminogen - Related Videos
05:37
Intravitreous Injection for Establishing Ocular Diseases Model
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Cited by 36 •
2007
Intravitreous injection is a widely used technique in visual sciences research for ocular diseases or as direct application of local treatment. This video demonstrated a protocol for intravitreous injection using a 1ml syringe with glass pipette. Useful tips about avoiding massive bleeding and lens damage are given.
04:34
Measuring Microglial Uptake of Intravitreally-Delivered Fluorescent Particles Using Flow Cytometry
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2025
This video demonstrates flow cytometry for measuring microglial uptake of fluorescent bioparticles after intravitreal delivery in a mouse eye. Flow cytometry detects live cells expressing CD11b and distinguishes phagocytic microglia cells exhibiting green fluorescence from other myeloid cells, including Ly6C-expressing monocytes and Ly6G-expressing neutrophils.
03:37
Intravitreal Injections in the Ovine Eye
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Cited by 1 •
2022
Intravitreal injections were performed in the sheep eye with the aim of delivering viral-mediated gene therapy to the retina.
03:57
Bacterial Endophthalmitis Mouse Model: A Method for Generating Bacterial Endophthalmitis via Intravitreal Injection of Bacterial Suspension into Eye of Mouse
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2025
This video describes a method for generating a mouse model that mimics bacterial endophthalmitis – an intraocular infection resulting from a bacterial invasion into the eye’s vitreous cavity – via intravitreal injection of a bacterial suspension into the eye. This mouse model helps study the disease mechanism and evaluate therapeutics for it.
06:20
Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model
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Cited by 4 •
2023
Methods for simple, rapid induction of a back pain model in mice are provided here using an intraligament injection of urinary plasminogen activator.
FAQs
The key mechanistic step occurs after administration, when local plasminogen activators convert the inactive precursor into plasmin within the vitreous environment. This creates a localized source of fibrinolytic activity rather than introducing the active enzyme directly. The resulting plasmin can act on fibrin and extracellular-matrix components, making local activation central to the proposed tissue-remodeling effect.
Impaired fibrinolysis can allow fibrin or related extracellular-matrix material to persist in ocular tissues. Supplying plasminogen locally may provide additional substrate for the eye’s plasminogen activators, potentially restoring part of the pathway that generates plasmin. This rationale is particularly relevant to ocular manifestations associated with plasminogen deficiency, where localized enzyme replacement is being investigated.
The generated plasmin may break down fibrin and other extracellular-matrix components associated with abnormal ocular deposits or adhesions. This matters because such material can contribute to persistent tissue associations that require remodeling. Studying these effects helps researchers determine whether localized fibrinolytic activity can modify abnormal ocular material without relying on a broadly distributed treatment.
The approach begins with administration of plasminogen into the vitreous cavity, followed by evaluation of how local activators generate plasmin and how the ocular environment responds. Investigators can then examine effects on fibrin, extracellular-matrix material, deposits, or adhesions. The central experimental feature is confined delivery within the eye rather than a systemic route.
Researchers may investigate intravitreal plasminogen in ocular conditions linked to impaired fibrinolysis, including manifestations associated with plasminogen deficiency. Its use as a study approach allows investigators to test whether supplying the precursor locally influences abnormal deposits or adhesions. The focus is not only on treatment potential, but also on understanding how fibrinolytic pathways contribute to ocular disease.
Localized replacement can help reveal whether increasing precursor availability inside the eye changes the formation or persistence of fibrin-rich deposits and extracellular-matrix adhesions. Because delivery is targeted to the vitreous cavity, the research framework also helps assess local biological effects while limiting systemic exposure. These observations may clarify how enzyme replacement influences tissue remodeling in ocular disease.