Sonication Fluid Analysis
Sonication Fluid Analysis - Related Videos
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
10:23
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
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Cited by 2 •
2017
A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies.
07:51
Synovial Fluid Analysis to Identify Osteoarthritis
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Cited by 6 •
2022
Synovial fluid analysis under transmitted, polarized, and compensated light microscopy is used to evaluate the inflammatory or non-inflammatory nature of a sample through simple steps. It is particularly useful in osteoarthritis to detect calcium crystals and identify a more severe subset of osteoarthritis.
FAQs
During ultrasonic treatment, cavitation produces physical forces in the surrounding fluid that disturb material attached to the removed implant. These forces can disrupt biofilms, which may otherwise retain bacteria or fungi on the surface, and release the organisms into the fluid. Testing that fluid therefore provides access to microorganisms that might not be recovered efficiently from the implant itself.
Biofilm-associated microorganisms can remain attached to an implant surface, making them difficult to detect through conventional tissue sampling alone. Sonication fluid analysis targets this surface-associated population by transferring released bacteria or fungi into a testable fluid. This distinction matters because identifying organisms linked to the implant can strengthen the microbiological assessment of a suspected infection.
Conventional tissue samples examine microorganisms recovered from surrounding tissue, whereas sonication fluid analysis examines organisms released from the removed implant surface. The two approaches access different microbial locations. Because sonication can disrupt surface biofilms, it may reveal bacteria or fungi that are difficult to recover from tissue samples, providing complementary information during evaluation of implant-associated infection.
The process begins after a medical implant has been removed and fluid has been collected following ultrasonic treatment. The resulting sonication fluid is then subjected to microbiological evaluation, using culture, molecular testing, or both. This workflow converts microorganisms detached from the implant surface into a sample that can be analyzed for evidence of infection.
Sonication fluid can undergo culture or molecular testing to investigate microorganisms released from the implant. Culture supports recovery of bacteria or fungi in the fluid, while molecular testing examines microbial material through a different analytical route. Using these options allows the sample to provide microbiological evidence that may help characterize an implant-associated infection.
The method is particularly relevant when clinicians evaluate suspected infections involving removed implants, including orthopedic prostheses. Its findings can help detect biofilm-associated bacteria or fungi, support diagnosis, and guide antimicrobial selection. By clarifying which microorganisms are associated with the implant, the results may also contribute to broader clinical management decisions.