Vessel Ligation
Vessel Ligation - Related Videos
07:34
DNA Ligation Reactions
0 Views •
2023
In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication. In the laboratory, DNA ligase is used during molecular cloning to join DNA fragments of inserts with vectors – carrier DNA molecules that will replicate target fragments in host organisms. This video provides an...
10:01
En Face Preparation of Mouse Blood Vessels
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Cited by 20 •
2017
A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.
11:53
Cecal Ligation Puncture Procedure
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Cited by 200 •
2011
The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.
05:47
Modified Technique for Coronary Artery Ligation in Mice
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Cited by 8 •
2013
The surgical procedure used to induce experimental myocardial infarction in mice begins with left thoracotomy between the third and the fourth ribs in order to visualize the anterior surface of the heart and left lung. The left coronary artery is ligated, the chest is closed and the mouse is allowed to recover spontaneously.
13:10
Stability of Floating Vessels
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2023
Source: Alexander S Rattner and Kevin Rao Li Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA The objective of this experiment is to demonstrate the phenomenon of stability of floating vessels - the ability to self-right when rolled over to the side by some external force. Careful design of hull shapes and internal mass distribution enables seagoing vessels to be stable with low drafts (submerged depth of hull), improving vessel...
FAQs
Closing the lumen interrupts blood flow through the treated segment, reducing the movement of blood into the operative field. This creates a controlled area in which surrounding tissues can be managed more safely. The closure may be produced by tying, clipping, or sealing the vessel, but the essential result is a sustained interruption of flow.
A secure closure maintains the intended interruption of flow through the treated segment. If the closure does not hold, blood may continue moving through that segment, reducing the effectiveness of hemostasis or vessel isolation. Procedural success therefore depends not only on placing a ligature, clip, or seal, but also on achieving reliable, lasting occlusion.
The treated vessel must be managed without unnecessarily compromising adjacent tissues or circulation that should remain available. This balance allows the targeted segment to be occluded while preserving the surrounding operative field and broader blood supply. It is particularly relevant when the procedure isolates one damaged or diseased vessel rather than affecting surrounding circulation.
The procedure must close the selected tubular structure, produce secure lumen occlusion, and protect nearby tissues and circulation. Depending on the surgical situation, closure may be achieved by tying, clipping, or sealing. These considerations connect the technical step with its intended outcome: controlling flow while allowing surrounding tissues to be managed safely.
In medicine, it can help isolate damaged or diseased vessels so the affected segment is separated from ongoing circulation. It also appears in surgical sterilization and in procedures for selected vascular abnormalities. These uses share a focus on deliberately interrupting flow in a defined structure, while the specific clinical purpose depends on the vessel and the operation.
In surgical sterilization, closing a selected tubular structure prevents normal passage through that structure by creating an occluded segment. The relevant effect is not general control of operative bleeding, but intentional interruption of transport through the treated pathway. This illustrates how the same closure principle can serve a planned sterilization procedure as well as intraoperative hemostasis.