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En Face Preparation of Mouse Blood Vessels
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En Face Preparation of Mouse Blood Vessels

En Face Preparation of Mouse Blood Vessels

DOI:
10.3791/55460-v

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10:01 min

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May 19, 2017

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, , ,
1Department of Cardiology, Division of Internal Medicine,University of Texas MD Anderson Cancer Center, 2Department of Radiation Oncology,University of Texas MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 00:53Left Partial Carotid Artery Ligation
  • 03:29En Face Immunostaining
  • 08:09Results: Representative En Face Immunofluorescent Imaging
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.

Tags

Keywords: En FaceMouse Blood VesselsAortaCarotid ArteryImmunostainingVascular CellsCarotid Artery LigationSurgical ProcedureAnesthetized MouseSalivary GlandsInternal Carotid ArteryExternal Carotid ArteryLigationOccipital ArterySuturesThoracic Cavity

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