Cell Surface Dynamics
Cell Surface Dynamics - Related Videos
06:31
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
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Cited by 10 •
2015
The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.
10:43
Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
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Cited by 3 •
2019
We describe an imaging approach for the determination of the average oligomeric state of mEGFP-tagged-receptor oligomers induced by ligand binding in the plasma membrane of living cells. The protocol is based on Total Internal Reflection Fluorescence (TIRF) microscopy combined with Number and Brightness (N&B) analysis.
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cited by 8 •
2020
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.
09:24
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
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Cited by 6 •
2020
The goal of this protocol is to show how to use Lattice Light-Sheet Microscopy to four-dimensionally visualize surface receptor dynamics in live cells. Here T cell receptors on CD4+ primary T cells are shown.
01:21
Cell-surface Signaling
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2019
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects. Variety of Receptor Families
FAQs
Lateral diffusion redistributes membrane components within the plasma membrane, whereas endocytosis removes receptors, channels, lipids, or adhesion molecules from the surface. Exocytosis returns or adds components to the membrane. Together, these processes alter the local composition of neuronal membranes and regulate which signaling molecules remain available at synapses.
The cortical cytoskeleton helps organize and remodel molecules at the inner surface of the plasma membrane. Its interactions with receptors, ion channels, adhesion molecules, and membrane structures can influence their redistribution. In neurons, this organization supports controlled changes in membrane composition rather than unrestricted movement across the cell surface.
Changing the number and location of receptors and ion channels at neuronal membranes changes how synapses respond to neurotransmitters and regulate electrical activity. Cell surface dynamics therefore provides a mechanism for adjusting synaptic signaling over time. Such changes can contribute to synaptic plasticity, allowing neural connections to adapt their functional responses.
Examining the redistribution of receptors, ion channels, lipids, and adhesion molecules can reveal how membrane organization changes during neuronal signaling. Tracking the balance between surface retention, internalization, and return to the membrane helps connect molecular movement with altered neurotransmission. This provides a framework for interpreting how synaptic responses are regulated.
During neural development, membrane components must be organized and remodeled as neurons establish and modify their interactions with surrounding cells. Changes in adhesion molecules and other surface components can influence how neuronal membranes respond to their environment. Studying these processes helps relate membrane remodeling to the formation and adaptation of neural connections.
Disrupted membrane trafficking or abnormal regulation of receptors can change which signaling components are present at neuronal surfaces. That imbalance may interfere with neurotransmission and the adjustment of synaptic responses. Cell surface dynamics research provides a way to investigate how molecular defects in membrane organization could contribute to disorders involving neuronal communication.