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Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
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Biochemistry
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JoVE Journal Biochemistry
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

DOI:
10.3791/59914-v

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09:24 min

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January 30, 2020

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,
1Committee on Cancer Biology,The University of Chicago, 2Pritzker School of Molecular Engineering,The University of Chicago

Chapters

  • 00:04Introduction
  • 01:22Prepare Cells
  • 03:40Conducting LLSM Daily Alignment
  • 05:13Setting up Cells with LLSM
  • 07:03Results: Four-dimensional Visualization of Surface T-cell Receptor Dynamics
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to show how to use Lattice Light-Sheet Microscopy to four-dimensionally visualize surface receptor dynamics in live cells. Here T cell receptors on CD4+ primary T cells are shown.

Tags

Keywords: Four-dimensional ImagingLattice Light-sheet MicroscopyLive Cell ImagingReal-time TrackingMolecular DynamicsT-cell ReceptorAntigen-presenting CellsSample PreparationCell Labeling

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