Confocal Ophthalmoscopy
Confocal Ophthalmoscopy - Related Videos
10:48
Imaging Biological Samples with Optical and Confocal Microscopy
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2023
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
11:21
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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Cited by 24 •
2013
Photoacoustic ophthalmology (PAOM), an optical-absorption-based imaging modality, provides the complementary evaluation of the retina to the currently available ophthalmic imaging technologies. We report the using of PAOM integrated with spectral-domain optical coherence tomography (SD-OCT) for simultaneous multimodal retinal imaging in rats.
06:19
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
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Cited by 5 •
2017
This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively.
07:27
Live Confocal Imaging of Developing Arabidopsis Flowers
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Cited by 16 •
2017
Live confocal imaging provides biologists with a powerful tool to study development. Here, we present a detailed protocol for the live confocal imaging of developing Arabidopsis flowers.
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
FAQs
The pinhole aperture rejects light originating outside the focused focal region, reducing background from surrounding tissue. This improves image contrast and helps separate signals from different retinal depths. As a result, researchers can examine retinal layers and optic-nerve structures more clearly than they could when out-of-focus light obscures fine anatomical features.
Optical sectioning provides depth-resolved views rather than combining signals from multiple tissue planes into one image. This separation helps investigators examine individual retinal layers, nerve fiber regions, and related structural features. In neuroscience studies, distinguishing these depths supports more precise assessment of retinal neurons and changes associated with visual-system pathology.
A focused light beam concentrates illumination on a defined tissue location, while scanning moves that focus across the examined region. Together with pinhole-based rejection of out-of-focus light, this process supports high-resolution imaging with improved contrast. The resulting images are useful when small structural differences in the retina or optic nerve are important.
The imaging process centers on directing a focused beam into the eye, scanning the relevant retinal or optic-nerve region, and using a pinhole aperture to exclude out-of-focus light. The collected image can then represent tissue structure at defined depths. This workflow produces depth-resolved information for examining living visual-system tissues.
Repeated imaging allows researchers to compare the same visual-system structures over time rather than relying on a single observation. Longitudinal comparisons can reveal structural changes in retinal layers, nerve fiber regions, or microvasculature. This makes the method useful for investigating disease progression and for relating ocular changes to neurodegenerative processes.
In neuroscience, the technique supports analysis of retinal neurons, nerve fiber layers, and microvascular changes in living eyes. Researchers can investigate glaucoma, retinal disease, and neurodegeneration while also examining whether ocular biomarkers correspond with broader nervous-system function. Its structural measurements therefore connect accessible eye-based observations with visual and neurological research questions.