Larva Imaging Technique
Larva Imaging Technique - Related Videos
02:24
A Technique to Quantify Fungal Infection in Zebrafish Larvae
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2025
This video demonstrates a method to quantify fungal infection in zebrafish larvae. Fungal spores are microinjected into the larval hindbrain ventricle, and the larvae are incubated to allow infection development. Infected larvae are harvested at defined intervals, and infection progress is assessed by enumerating colony-forming units.
10:45
Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
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Cited by 33 •
2012
The rapid development, small size and transparency of zebrafish are tremendous advantages for the study of innate immune control of infection1-4. Here we demonstrate techniques for infecting zebrafish larvae using the fungal pathogen Candida albicans by microinjection, methodology recently used to implicate phagocyte NADPH oxidase activity in control of fungal dimorphism5.
02:58
A zWEDGI Technique to Visualize Fungal Pathogen-Infected Zebrafish Larvae
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2025
The video demonstrates a zebrafish wounding and entrapment device technique for observing fluorescently labeled fungal pathogen infection in zebrafish larvae. Initial infection attracts macrophages, and as infection persists, neutrophil recruitment progresses at the injection site.
03:54
A Technique to Establish a Bacterial Infection Model in Insect Larvae
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2025
This video demonstrates a method to establish a Mycobacterium bovis infection model in the larvae of the greater wax moth, Galleria mellonella. A bioluminescent strain of Mycobacterium bovis BCG is injected into the hemolymph of the larva to establish infection, and the bioluminescence is measured at predefined intervals to study the progress of infection.
17:51
In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
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Cited by 21 •
2010
This protocol describes a reliable method for anesthetization and imaging of intact Drosophila melanogaster larvae. We have utilized the volatile anesthetic desflurane to allow for repetitive imaging at sub-cellular resolution and re-identification of structures for up to a few days1.
FAQs
Transmitted light can show overall larval anatomy, whereas fluorescence makes labeled neurons, axons, or neural circuits easier to follow. Time-lapse imaging adds the dimension of change, allowing researchers to track development, neural activity, or behavioral responses over time. Combining these approaches helps relate visible cellular features to evolving nervous-system structure and function.
Immobilization limits movement during image acquisition, making neural structures and activity easier to observe consistently. Controlled conditions help researchers distinguish biological changes from variation caused by shifting position or unstable imaging conditions. This is especially important when following the same labeled neurons, axons, or circuits across time and comparing responses between experimental groups.
Because the larval nervous system remains within its intact anatomy, imaging can connect cellular organization with brain function in the same organism. Researchers can examine how developing neurons and axons relate to neural activity or behavioral responses, rather than considering structure in isolation. This supports interpretation of how nervous-system changes may influence function.
A typical workflow begins by placing larvae under controlled conditions and immobilizing them for stable observation. Researchers then select transmitted light, fluorescence, or time-lapse imaging according to the feature being studied, such as anatomy, labeled neurons, axons, or activity. Images are subsequently examined over time to relate structural or functional changes to responses.
This approach is useful when investigators need to examine neurodevelopment, sensory processing, neuronal injury, or disease mechanisms in living larvae. It also supports studies of how genetic or pharmacological manipulations affect the nervous system. The intact preparation allows these questions to be examined while cellular changes remain connected to neural function or behavioral responses.
Imaging can show whether an intervention changes nervous-system structure or function, including features associated with labeled neurons, axons, neural circuits, activity, or behavioral responses. Time-lapse observations may further indicate when changes emerge during development or after treatment. These outcomes help researchers evaluate mechanisms of disease, injury, or altered neural development.